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Toxins (Basel). 2018 Feb 17;10(2). pii: E85. doi: 10.3390/toxins10020085.

Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study.

Author information

1
School of Medicine, Universidad Cooperativa de Colombia, Sede Medellín, Street 50 A N° 41-20, Medellín 050010, Colombia. juan.quintanac@ucc.edu.co.
2
School of Medicine, Universidad Cooperativa de Colombia, Sede Medellín, Street 50 A N° 41-20, Medellín 050010, Colombia. leidy.vargasmu@campusucc.edu.co.
3
Malaria Group, School of Medicine, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, Colombia. cesars79@gmail.com.
4
Ophidism/Scorpionism Program, Food and Pharmaceutical Sciences Faculty, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, Colombia. sebastian.estrada@udea.edu.co.
5
Reproduction Group, School of Medicine, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, Colombia. julio.bueno@yahoo.com.
6
Ophidism/Scorpionism Program, Food and Pharmaceutical Sciences Faculty, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, Colombia. juan.alarcon@udea.edu.co.

Abstract

The Colombian rattlesnake Crotalus durissus cumanensis is distributed in three geographic zones of the country: the Atlantic Coast, the upper valley of the Magdalena River, and the eastern plains of the Colombian Orinoquía. Its venom induces neurological symptoms, such as eyelid ptosis, myasthenic facies, and paralysis of the respiratory muscles, which can lead to death. Identification and analysis of C. d. cumanensis showed nine groups of proteins responsible for the neurotoxic effect, of which the crotoxin complex was the most abundant (64.71%). Immunorecognition tests of C. d. cumanensis showed that the use of a commercial antivenom manufactured in Mexico resulted in immunoreactivity.

KEYWORDS:

C. d. cumanensis venom; HPLC-nESI-MS/MS; antivenom; peptide identification

PMID:
29462980
PMCID:
PMC5848186
DOI:
10.3390/toxins10020085
[Indexed for MEDLINE]
Free PMC Article

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