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Biosensors (Basel). 2019 Jan 2;9(1). pii: E7. doi: 10.3390/bios9010007.

Characterization and Inkjet Printing of an RNA Aptamer for Paper-Based Biosensing of Ciprofloxacin.

Author information

1
Department of Biology, Technische Universität Darmstadt, 64287 Darmstadt, Germany. jaeger.jea@bio.tu-darmstadt.de.
2
Department of Biology, Technische Universität Darmstadt, 64287 Darmstadt, Germany. groher@bio.tu-darmstadt.de.
3
Institute for Printing Science and Technology, Technische Universität Darmstadt, 64289 Darmstadt, Germany. stamm@idd.tu-darmstadt.de.
4
Institute for Printing Science and Technology, Technische Universität Darmstadt, 64289 Darmstadt, Germany. spiehl@idd.tu-darmstadt.de.
5
Department of Biology, Technische Universität Darmstadt, 64287 Darmstadt, Germany. braunj@bio.tu-darmstadt.de.
6
Institute for Printing Science and Technology, Technische Universität Darmstadt, 64289 Darmstadt, Germany. doersam@idd.tu-darmstadt.de.
7
Department of Biology, Technische Universität Darmstadt, 64287 Darmstadt, Germany. bsuess@bio.tu-darmstadt.de.

Abstract

The excessive use of antibiotics in food-producing animals causes a steady rise of multiple antibiotic resistance in foodborne bacteria. Next to sulfonamides, the most common antibiotics groups are fluoroquinolones, aminoglycosides, and ß-lactams. Therefore, there is a need for a quick, efficient, and low-cost detection procedure for antibiotics. In this study, we propose an inkjet-printed aptamer-based biosensor developed for the detection of the fluoroquinolone ciprofloxacin. Due to their extraordinary high affinity and specificity, aptamers are already widely used in various applications. Here we present a ciprofloxacin-binding RNA aptamer developed by systematic evolution of ligands by exponential enrichment (SELEX). We characterized the secondary structure of the aptamer and determined the KD to 36 nM that allow detection of antibiotic contamination in a relevant range. We demonstrate that RNA aptamers can be inkjet-printed, dried, and resolved while keeping their functionality consistently intact. With this proof of concept, we are paving the way for a potential range of additional aptamer-based, printable biosensors.

KEYWORDS:

aptamer; biosensor; ciprofloxacin; detection; fluorescence; printing

PMID:
30609709
PMCID:
PMC6468496
DOI:
10.3390/bios9010007
[Indexed for MEDLINE]
Free PMC Article

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