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Biomolecules. 2018 Sep 4;8(3). pii: E87. doi: 10.3390/biom8030087.

Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in an N-Acyl Homoserine Lactone (AHL)-Based Escherichia coli Biosensor.

Author information

1
Institute of Plant Biology and Biotechnology, University of Münster, Schlossplatz 8, 48143 Münster, Germany. qxf200459420@163.com.
2
Institute of Plant Biology and Biotechnology, University of Münster, Schlossplatz 8, 48143 Münster, Germany. jana.emich@wwu.de.
3
Institute of Human Genetics, University Hospital Münster, Vesaliusweg 12-14, 48149 Münster, Germany. jana.emich@wwu.de.
4
Institute of Plant Biology and Biotechnology, University of Münster, Schlossplatz 8, 48143 Münster, Germany. F.M.Goycoolea@leeds.ac.uk.
5
School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK. F.M.Goycoolea@leeds.ac.uk.

Abstract

New approaches to deal with drug-resistant pathogenic bacteria are urgent. We studied the antibacterial effect of chitosans against an Escherichia coli quorum sensing biosensor reporter strain and selected a non-toxic chitosan to evaluate its quorum sensing (QS) inhibition activity and its effect on bacterial aggregation. To this end, chitosans of varying degree of acetylation (DA) (12 to 69%) and molecular weight (Mw) (29 to 288 kDa) were studied. Only chitosans of low DA (~12%) inhibited bacterial growth, regardless of their Mw. A chitosan with medium degree of polymerization (named MDP) DA30, with experimental DA 42% and Mw 115 kDa was selected for further QS inhibition and scanning electron microscopy (SEM) imaging studies. MDP DA30 chitosan exhibited QS inhibition activity in an inverse dose-dependent manner (≤12.5 µg/mL). SEM images revealed that this chitosan, when added at low concentration (≤30.6 µg/mL), induced substantial bacterial aggregation, whereas at high concentration (234.3 µg/mL), it did not. Aggregation explains the QS inhibition activity as the consequence of retardation of the diffusion of N-acylated homoserine lactones (AHLs).

KEYWORDS:

antibacterial activity; chitosan; quorum sensing; quorum sensing inhibition

PMID:
30181497
PMCID:
PMC6164843
DOI:
10.3390/biom8030087
[Indexed for MEDLINE]
Free PMC Article

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