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Molecules. 2019 Apr 15;24(8). pii: E1484. doi: 10.3390/molecules24081484.

Use of Physcion to Improve Atopic Dermatitis-Like Skin Lesions through Blocking of Thymic Stromal Lymphopoietin.

Author information

1
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea. pdmoon@khu.ac.kr.
2
Center for Converging Humanities, Kyung Hee University, Seoul 02447, Korea. pdmoon@khu.ac.kr.
3
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea. nrhan@khu.ac.kr.
4
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea. mcjin21@naver.com.
5
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Korea. lov4all@hanmail.net.
6
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Korea. ymshappy@nate.com.
7
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Korea. khjhj0999@hanmail.net.
8
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Korea. sjsdlsrk@naver.com.
9
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Korea. sensedatum@naver.com.
10
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Korea. aidenjee@naver.com.
11
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea. hmkim@khu.ac.kr.
12
Department of Food Science & Technology and Research Institute for Basic Science, Hoseo University, Chungnam 31499, Korea. hjjeong@hoseo.edu.

Abstract

Physcion is well known for the treatment of carcinoma. However, the therapeutic effect of physcion on atopic dermatitis (AD) through the inhibition of thymic stromal lymphopoietin (TSLP) level remains largely unknown. In this study, we investigated the anti-AD effect of physcion using HMC-1 cells, splenocytes, and a murine model. Treatment with physcion decreased production and mRNA expression levels of TSLP, IL-6, TNF-ɑ, and IL-1β in activated HMC-1 cells. Physcion reduced the expression levels of RIP2/caspase-1 and phospho (p)ERK/pJNK/pp38 in activated HMC-1 cells. Physcion suppressed the expression levels of pIKKβ/NF-κB/pIkB in activated HMC-1 cells. Moreover, physcion attenuated the production levels of TSLP, IL-4, IL-6, TNF-, and IFN-γ from activated splenocytes. Oral administration of physcion improved the severity of 2,4-dinitrochlorobenzene-induced AD-like lesional skin through reducing infiltration of inflammatory cells and mast cells, and the protein and mRNA levels of TSLP, IL-4, and IL-6 in the lesional skin tissues. Physcion attenuated histamine, IgE, TSLP, IL-4, IL-6, and TNF- levels in serum. In addition, physcion inhibited caspase-1 activation in the lesional skin tissues. These findings indicate that physcion could ameliorate AD-like skin lesions by inhibiting TSLP levels via caspase-1/MAPKs/NF-kB signalings, which would provide experimental evidence of the therapeutic potential of physcion for AD.

KEYWORDS:

atopic dermatitis; caspase-1; mast cells; physcion; thymic stromal lymphopoietin

PMID:
30991764
PMCID:
PMC6514936
DOI:
10.3390/molecules24081484
[Indexed for MEDLINE]
Free PMC Article

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