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Materials (Basel). 2016 Mar 30;9(4). pii: E249. doi: 10.3390/ma9040249.

Rapid Assay to Assess Bacterial Adhesion on Textiles.

Author information

1
Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen 9014, Switzerland. sabrina.schmidt@empa.ch.
2
Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen 9014, Switzerland. philipp.stiefel@empa.ch.
3
Laboratory for Advanced Fibers, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen 9014, Switzerland. patrick.rupper@empa.ch.
4
Sanitized AG, Burgdorf 3400, Switzerland. heinz.katzenmeier@bluewin.ch.
5
Swissatest Testmaterials AG, St. Gallen 9015, Switzerland. caroline.amberg@swissatest.ch.
6
Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen 9014, Switzerland. katharina.maniura@empa.ch.
7
Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen 9014, Switzerland. qun.ren@empa.ch.

Abstract

Textiles are frequently colonized by microorganisms leading to undesired consequences like hygienic problems. Biocidal coatings often raise environmental and health concerns, thus sustainable, biocide-free coatings are of interest. To develop novel anti-adhesive textile coatings, a rapid, reliable, and quantitative high-throughput method to study microbial attachment to fabrics is required, however currently not available. Here, a fast and reliable 96-well plate-based screening method is developed. The quantification of bacterial adhesion is based on nucleic acid staining by SYTO9, with Pseudomonas aeruginosa and Staphylococcus aureus as the model microorganisms. Subsequently, 38 commercially available and novel coatings were evaluated for their anti-bacterial adhesion properties. A poly(l-lysine)-g-poly(ethylene glycol) coating on polyester textile substratum revealed an 80% reduction of bacterial adhesion. Both the coating itself and the anti-adhesive property were stable after 20 washing cycles, confirmed by X-ray analysis. The assay provides an efficient tool to rapidly screen for non-biocidal coatings reducing bacterial attachment.

KEYWORDS:

antifouling; bacterial adhesion; biofilm; microtiter plate; textile coating

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