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Int J Mol Sci. 2014 Jun 16;15(6):10855-67. doi: 10.3390/ijms150610855.

Bacterial cellulose membranes used as artificial substitutes for dural defection in rabbits.

Author information

1
Department of Neurosurgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Yishan Road 600, Shanghai 200233, China. aceorange@shsmu.edu.cn.
2
School of Perfume and Aroma Technology, Shanghai Institute of Technology, Haiquan Road 100, Shanghai 201418, China. maxia0927@gmail.com.
3
Department of Neurosurgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Yishan Road 600, Shanghai 200233, China. chenshiwen@126.com.
4
School of Life Sciences and Biotechnology, Shanghai Jiaotong University, Dongchuan Road 800, Shanghai 200240, China. taomeifeng0421@gmail.com.
5
Department of Neurosurgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Yishan Road 600, Shanghai 200233, China. yuanlutao001@gmail.com.
6
Department of Neurosurgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Yishan Road 600, Shanghai 200233, China. jingyao0728@gmail.com.

Abstract

To improve the efficacy and safety of dural repair in neurosurgical procedures, a new dural material derived from bacterial cellulose (BC) was evaluated in a rabbit model with dural defects. We prepared artificial dura mater using bacterial cellulose which was incubated and fermented from Acetobacter xylinum. The dural defects of the rabbit model were repaired with BC membranes. All surgeries were performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering. All animals were humanely euthanized by intravenous injection of phenobarbitone, at each time point, after the operation. Then, the histocompatibility and inflammatory effects of BC were examined by histological examination, real-time fluorescent quantitative polymerase chain reaction (PCR) and Western Blot. BC membranes evenly covered the surface of brain without adhesion. There were seldom inflammatory cells surrounding the membrane during the early postoperative period. The expression of inflammatory cytokines IL-1β, IL-6 and TNF-α as well as iNOS and COX-2 were lower in the BC group compared to the control group at 7, 14 and 21 days after implantation. BC can repair dural defects in rabbit and has a decreased inflammatory response compared to traditional materials. However, the long-term effects need to be validated in larger animals.

PMID:
24937688
PMCID:
PMC4100185
DOI:
10.3390/ijms150610855
[Indexed for MEDLINE]
Free PMC Article

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