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Materials (Basel). 2016 Jun 15;9(6). pii: E468. doi: 10.3390/ma9060468.

Microbially Induced Calcite Precipitation Employing Environmental Isolates.

Author information

1
Department of Civil Engineering, Hongik University, 94, Wausan-ro, Mapo-gu, Seoul 04066, Korea. rmsdbrto@naver.com.
2
Department of Civil Engineering, Hongik University, 94, Wausan-ro, Mapo-gu, Seoul 04066, Korea. geotech@hongik.ac.kr.

Abstract

In this study, five microbes were employed to precipitate calcite in cohesionless soils. Four microbes were selected from calcite-precipitating microbes isolated from calcareous sand and limestone cave soils, with Sporosarcina pasteurii ATCC 11859 (standard strain) used as a control. Urease activities of the four microbes were higher than that of S. pasteurii. The microbes and urea-CaCl₂ medium were injected at least four times into cohesionless soils of two different relative densities (60% and 80%), and the amount of calcite precipitation was measured. It was found that the relative density of cohesionless soils significantly affects the amount of calcite precipitation and that there is a weak correlation between urease activity and calcite precipitation.

KEYWORDS:

MICP; calcite precipitation; environmental isolates; microbe; urease activity

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