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Plants (Basel). 2019 Mar 31;8(4). pii: E83. doi: 10.3390/plants8040083.

Karyological Analysis and DNA Barcoding of Pompia Citron: A First Step toward the Identification of Its Relatives.

Author information

1
Dipartimento di AGRARIA Research Unit SACEG, University of Sassari, 07100 Sassari, Italy. graziaviglietti@gmail.com.
2
Laboratory of Genomics, Department of Agronomy Food Natural Resources Animals and Environment, University of Padova, 35020 Legnaro, Padova, Italy. giulio.galla@unipd.it.
3
Dipartimento di AGRARIA Research Unit SACEG, University of Sassari, 07100 Sassari, Italy. aporceddu@uniss.it.
4
Laboratory of Genomics, Department of Agronomy Food Natural Resources Animals and Environment, University of Padova, 35020 Legnaro, Padova, Italy. gianni.barcaccia@unipd.it.
5
Unite Mixte de Recherche Amelioration Genetique et Adaptation des Plantes (UMR Agap), Institut National de la Recherche Agronomique (INRA), F-20230 San Giuliano, France. franck.curk@inra.fr.
6
Unite Mixte de Recherche Amelioration Genetique et Adaptation des Plantes (UMR Agap), Institut National de la Recherche Agronomique (INRA), F-20230 San Giuliano, France. francois.luro@inra.fr.
7
Dipartimento di AGRARIA Research Unit SACEG, University of Sassari, 07100 Sassari, Italy. grazia@uniss.it.

Abstract

Pompia is a citrus fruit endemic of Sardinia, Italy, with an essential oil profile showing outstanding anti-inflammatory and anti-microbic properties. Despite its remarkable pharmaceutical potential, little taxonomic and genetic information is available for this species. We applied flow cytometry and classical cytogenetic techniques to assess the DNA content and to reconstruct the karyotype of several Pompia accessions. Molecular data from plastid DNA barcoding and nuclear DNA sequencing were used to study the genetic distance between Pompia and other citrus species. Flow cytometric estimates of DNA content and somatic chromosome counts suggest that Pompia is a regular diploid Citrus species. DNA polymorphisms of nuclear and chloroplast markers allowed us to investigate the genetic relationships between Pompia accessions and other Citrus species. Based on DNA polymorphism data we propose that Pompia is a very recent interspecific hybrid generated by a cross between C. aurantium (as seed bearer) and C. medica (as pollen donor). Our findings pave the way for further and more specific investigations of local Pompia germplasm resources that may help the preservation and valorisation of this valuable citrus fruit tree.

KEYWORDS:

DNA content; ITS sequence; Karyotype; psbA-trnH sequence; trnL intron sequence

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