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Bioelectrochemistry. 2018 Aug;122:183-190. doi: 10.1016/j.bioelechem.2018.04.006. Epub 2018 Apr 10.

Different permeabilization patterns of splenocytes and thymocytes to combination of pulsed electric and magnetic field treatments.

Author information

1
Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Vilnius, Lithuania. Electronic address: vitalij.novickij@vgtu.lt.
2
State Research Institute Centre for Innovative Medicine, Department of Immunology, Vilnius, Lithuania.
3
Laboratory of Biodeterioration Research, Nature Research Centre, Vilnius, Lithuania.
4
Institute of Biosciences, Life Sciences Centre, Vilnius University, Vilnius, Lithuania.
5
Laboratory of Mycology, Nature Research Centre, Vilnius, Lithuania.
6
Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Vilnius, Lithuania.

Abstract

Genetic manipulation of T cells is frequently inefficient, however, when combined with physical methods (i.e. electroporation) a promising alliance with immunotherapy can be formed. This study presents new data on permeabilization of murine thymocytes and splenocytes as a T cell model using pulsed electric (PEF) and electromagnetic field (EMF). The 300ns, 500ns, 2μs and 100μs pulse bursts in a broad range of PEF 0-8kV/cm were applied separately and in combination with 3.3T, 0.2kV/cm EMF pulses. The permeabilization efficiency was evaluated using fluorescent dye (YO-PRO-1) and flow cytometry. It was shown that a >14% increase in thymocytes permeabilization is achieved when electroporation is applied in combination with EMF, however splenocytes responded in a different manner - a statistically significant (P<0.05) reduction in permeabilization was observed. The cytokine secretion patterns were mainly unaltered independently on the applied treatment parameters determined by secretion of IFNγ, IL-4 and IL-17 - the main cytokines of Th1, Th2 and Th17 cells. The results of this study are useful for development of pulsed power protocols for effective genetic modification of T cells.

KEYWORDS:

Cytokines; Electroporation; Lymphocytes; Magnetoporation; Membrane permeabilization

[Indexed for MEDLINE]

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