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Bioelectrochemistry. 2018 Aug;122:183-190. doi: 10.1016/j.bioelechem.2018.04.006. Epub 2018 Apr 10.

Different permeabilization patterns of splenocytes and thymocytes to combination of pulsed electric and magnetic field treatments.

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Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Vilnius, Lithuania. Electronic address:
State Research Institute Centre for Innovative Medicine, Department of Immunology, Vilnius, Lithuania.
Laboratory of Biodeterioration Research, Nature Research Centre, Vilnius, Lithuania.
Institute of Biosciences, Life Sciences Centre, Vilnius University, Vilnius, Lithuania.
Laboratory of Mycology, Nature Research Centre, Vilnius, Lithuania.
Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Vilnius, Lithuania.


Genetic manipulation of T cells is frequently inefficient, however, when combined with physical methods (i.e. electroporation) a promising alliance with immunotherapy can be formed. This study presents new data on permeabilization of murine thymocytes and splenocytes as a T cell model using pulsed electric (PEF) and electromagnetic field (EMF). The 300ns, 500ns, 2μs and 100μs pulse bursts in a broad range of PEF 0-8kV/cm were applied separately and in combination with 3.3T, 0.2kV/cm EMF pulses. The permeabilization efficiency was evaluated using fluorescent dye (YO-PRO-1) and flow cytometry. It was shown that a >14% increase in thymocytes permeabilization is achieved when electroporation is applied in combination with EMF, however splenocytes responded in a different manner - a statistically significant (P<0.05) reduction in permeabilization was observed. The cytokine secretion patterns were mainly unaltered independently on the applied treatment parameters determined by secretion of IFNγ, IL-4 and IL-17 - the main cytokines of Th1, Th2 and Th17 cells. The results of this study are useful for development of pulsed power protocols for effective genetic modification of T cells.


Cytokines; Electroporation; Lymphocytes; Magnetoporation; Membrane permeabilization

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