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Int J Mol Sci. 2018 Jan 8;19(1). pii: E191. doi: 10.3390/ijms19010191.

Cloning and Functional Analysis of Phosphoethanolamine Methyltransferase Promoter from Maize (Zea mays L.).

Author information

1
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling 712100, China. gailiniu@nwafu.edu.cn.
2
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling 712100, China. maokailun@nwafu.edu.cn.
3
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling 712100, China. hanxianglong@nwafu.edu.cn.
4
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling 712100, China. Qincheng@nwafu.edu.cn.
5
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling 712100, China. zhanglixin@nwafu.edu.cn.
6
New Energy Department, School of Energy and Power Engineering, Jiangsu University, Zhenjiang 212013, China. abomohra@ujs.edu.cn.
7
Botany Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt. abomohra@ujs.edu.cn.
8
Pakistan Science Foundation, Islamabad 44000, Pakistan. ashrafbot@yahoo.com.

Abstract

Betaine, a non-toxic osmoprotectant, is believed to accumulate considerably in plants under stress conditions to maintain the osmotic pressure and promote a variety of processes involved in growth and development. Phosphoethanolamine N-methyltransferase (PEAMT), a key enzyme for betaine synthesis, is reported to be regulated by its upstream promoter. In the present investigation, by using the transgenic approach, a 1048 bp long promoter region of ZmPEAMT gene from Zea mays was cloned and functionally characterized in tobacco. Computational analysis affirmed the existence of abiotic stress responsive cis-elements like ABRE, MYC, HST, LST etc., as well as pathogen, wound and phytohormone responsive motifs. For transformation in tobacco, four 5'-deletion constructs of 826 bp (P2), 642 bp (P3), 428 bp (P4) and 245 bp (P5) were constructed from the 1048 bp (P1) promoter fragment. The transgenic plants generated through a single event exhibited a promising expression of GUS reporter protein in the leaf tissues of treated with salt, drought, oxidative and cold stress as well as control plants. The GUS expression level progressively reduced from P1 to P5 in the leaf tissues, whereas a maximal expression was observed with the P3 construct in the leaves of control plants. The expression of GUS was noted to be higher in the leaves of osmotically- or salt-treated transgenic plants than that in the untreated (control) plants. An effective expression of GUS in the transgenic plants manifests that this promoter can be employed for both stress-inducible and constitutive expression of gene(s). Due to this characteristic, this potential promoter can be effectively used for genetic engineering of several crops.

KEYWORDS:

functional analysis; maize; phosphoethanolamine N-methyltransferase gene (peamt); promoter

PMID:
29316727
PMCID:
PMC5796140
DOI:
10.3390/ijms19010191
[Indexed for MEDLINE]
Free PMC Article

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