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Nutr Metab Cardiovasc Dis. 2015 Jul;25(7):659-66. doi: 10.1016/j.numecd.2015.04.002. Epub 2015 Apr 22.

Centella asiatica and lipoic acid, or a combination thereof, inhibit monocyte adhesion to endothelial cells from umbilical cords of gestational diabetic women.

Author information

1
Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University, Chieti, Italy; Aging Research Center, Ce.S.I., "Gabriele d'Annunzio" University Foundation, Chieti, Italy.
2
Department of Medicine and Aging Sciences, "G. d'Annunzio" University, Chieti, Italy; Aging Research Center, Ce.S.I., "Gabriele d'Annunzio" University Foundation, Chieti, Italy.
3
Morningside Laboratory for Chemical Biology and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China.
4
CoreQuest Sagl, Manno, Ticino, Switzerland.
5
Department of Medicine and Aging Sciences, "G. d'Annunzio" University, Chieti, Italy.
6
Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University, Chieti, Italy; Aging Research Center, Ce.S.I., "Gabriele d'Annunzio" University Foundation, Chieti, Italy. Electronic address: n.dipietro@unich.it.

Abstract

BACKGROUND AND AIMS:

Diabetes mellitus is associated with inflammatory endothelial activation and increased vascular leukocyte adhesion molecule expression, both playing a prominent role in the development of vascular complications. Centella asiatica (CA) and Lipoic Acid (LA) have shown anti-inflammatory and anti-oxidant properties in a variety of experimental models; however, their action on human umbilical vein endothelial cells (HUVECs), chronically exposed to hyperglycemia and pro-inflammatory environment during pregnancy, is still unknown.

METHODS AND RESULTS:

In HUVECs from umbilical cords of gestational diabetic (GD) or healthy (C) women, both CA and LA affected tumor necrosis factor-α (TNF-α)-induced inflammation, being associated with a significant decrease in vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) expression (western blot) and exposure (flow cytometry), as well as monocyte-HUVECs interaction (adhesion assay). Notably, this was associated with a significant reduction of an index of nitro-oxidative stress, such as the intracellular peroxynitrite levels (fluorescence detection by cytometric analysis), Mitogen-Activated Protein kinase (p44/42 MAPK) expression/phosphorylation levels and Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB p65) cytoplasm-nucleus translocation (flow cytometry). Overall our results indicate that both CA and LA used separately, and even better when combined, are effective to reduce the inflammatory response in TNF-α-treated HUVECs. Notably, this was more significant in GD than in C-HUVECs and also evident at baseline.

CONCLUSION:

In conclusion, our in vitro study demonstrates that both CA and LA, or a combination thereof, are able to mitigate the potentially dangerous effects on the endothelium of chronic exposure to hyperglycemia in vivo.

KEYWORDS:

Centella asiatica; Endothelial dysfunction; Gestational diabetes; Inflammation; Lipoic Acid

PMID:
26026207
DOI:
10.1016/j.numecd.2015.04.002
[Indexed for MEDLINE]

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