Format

Send to

Choose Destination
Toxins (Basel). 2018 Feb 17;10(2). pii: E86. doi: 10.3390/toxins10020086.

Fusarium graminearum in Stored Wheat: Use of CO₂ Production to Quantify Dry Matter Losses and Relate This to Relative Risks of Zearalenone Contamination under Interacting Environmental Conditions.

Author information

1
Applied Mycology Group, Environment and AgriFood Theme, Cranfield University, Cranfield Beds. MK43 0AL, UK;. m.e.garcia-cela@cranfield.ac.uk.
2
Applied Mycology Group, Environment and AgriFood Theme, Cranfield University, Cranfield Beds. MK43 0AL, UK;. Elsa.Kiaitsi@cranfield.ac.uk.
3
Centre for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenz str. 20, A-3430 Tulln, Austria;. michael.sulyok@boku.ac.at.
4
Applied Mycology Group, Environment and AgriFood Theme, Cranfield University, Cranfield Beds. MK43 0AL, UK;. a.medinavaya@cranfield.ac.uk.
5
Applied Mycology Group, Environment and AgriFood Theme, Cranfield University, Cranfield Beds. MK43 0AL, UK;. n.magan@cranfield.ac.uk.

Abstract

Zearalenone (ZEN) contamination from Fusarium graminearum colonization is particularly important in food and feed wheat, especially during post-harvest storage with legislative limits for both food and feed grain. Indicators of the relative risk from exceeding these limits would be useful. We examined the effect of different water activities (aw; 0.95-0.90) and temperature (10-25 °C) in naturally contaminated and irradiated wheat grain, both inoculated with F. graminearum and stored for 15 days on (a) respiration rate; (b) dry matter losses (DML); (c) ZEN production and (d) relationship between DML and ZEN contamination relative to the EU legislative limits. Gas Chromatography was used to measure the temporal respiration rates and the total accumulated CO₂ production. There was an increase in temporal CO₂ production rates in wetter and warmer conditions in all treatments, with the highest respiration in the 25 °C × 0.95 aw treatments + F. graminearum inoculation. This was reflected in the total accumulated CO₂ in the treatments. The maximum DMLs were in the 0.95 aw/20-25 °C treatments and at 10 °C/0.95 aw. The DMLs were modelled to produce contour maps of the environmental conditions resulting in maximum/minimum losses. Contamination with ZEN/ZEN-related compounds were quantified. Maximum production was at 25 °C/0.95-0.93 aw and 20 °C/0.95 aw. ZEN contamination levels plotted against DMLs for all the treatments showed that at ca <1.0% DML, there was a low risk of ZEN contamination exceeding EU legislative limits, while at >1.0% DML, the risk was high. This type of data is important in building a database for the development of a post-harvest decision support system for relative risks of different mycotoxins.

KEYWORDS:

Fusarium graminearum; cereals; mycotoxins; respiration rates; risk; temperature; water activity

PMID:
29462982
PMCID:
PMC5848187
DOI:
10.3390/toxins10020086
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center