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Items: 1 to 20 of 22


The Impact of Oxidative Stress on Ribosomes: From Injury to Regulation.

Shcherbik N, Pestov DG.

Cells. 2019 Nov 2;8(11). pii: E1379. doi: 10.3390/cells8111379. Review.


Iron-dependent cleavage of ribosomal RNA during oxidative stress in the yeast Saccharomyces cerevisiae.

Zinskie JA, Ghosh A, Trainor BM, Shedlovskiy D, Pestov DG, Shcherbik N.

J Biol Chem. 2018 Sep 14;293(37):14237-14248. doi: 10.1074/jbc.RA118.004174. Epub 2018 Jul 18.


Endonucleolytic cleavage in the expansion segment 7 of 25S rRNA is an early marker of low-level oxidative stress in yeast.

Shedlovskiy D, Zinskie JA, Gardner E, Pestov DG, Shcherbik N.

J Biol Chem. 2017 Nov 10;292(45):18469-18485. doi: 10.1074/jbc.M117.800003. Epub 2017 Sep 22.


Inhibition of post-transcriptional steps in ribosome biogenesis confers cytoprotection against chemotherapeutic agents in a p53-dependent manner.

Sapio RT, Nezdyur AN, Krevetski M, Anikin L, Manna VJ, Minkovsky N, Pestov DG.

Sci Rep. 2017 Aug 22;7(1):9041. doi: 10.1038/s41598-017-09002-w.


One-step hot formamide extraction of RNA from Saccharomyces cerevisiae.

Shedlovskiy D, Shcherbik N, Pestov DG.

RNA Biol. 2017 Dec 2;14(12):1722-1726. doi: 10.1080/15476286.2017.1345417. Epub 2017 Jul 31.


Quantitative Northern Blot Analysis of Mammalian rRNA Processing.

Wang M, Pestov DG.

Methods Mol Biol. 2016;1455:147-57. doi: 10.1007/978-1-4939-3792-9_12.


Distinct types of translation termination generate substrates for ribosome-associated quality control.

Shcherbik N, Chernova TA, Chernoff YO, Pestov DG.

Nucleic Acids Res. 2016 Aug 19;44(14):6840-52. doi: 10.1093/nar/gkw566. Epub 2016 Jun 20.


Reduced expression of the mouse ribosomal protein Rpl17 alters the diversity of mature ribosomes by enhancing production of shortened 5.8S rRNA.

Wang M, Parshin AV, Shcherbik N, Pestov DG.

RNA. 2015 Jul;21(7):1240-8. doi: 10.1261/rna.051169.115. Epub 2015 May 20.


Two orthogonal cleavages separate subunit RNAs in mouse ribosome biogenesis.

Wang M, Anikin L, Pestov DG.

Nucleic Acids Res. 2014;42(17):11180-91. doi: 10.1093/nar/gku787. Epub 2014 Sep 4.


Separation of long RNA by agarose-formaldehyde gel electrophoresis.

Mansour FH, Pestov DG.

Anal Biochem. 2013 Oct 1;441(1):18-20. doi: 10.1016/j.ab.2013.06.008. Epub 2013 Jun 22.


Rapid cytoplasmic turnover of yeast ribosomes in response to rapamycin inhibition of TOR.

Pestov DG, Shcherbik N.

Mol Cell Biol. 2012 Jun;32(11):2135-44. doi: 10.1128/MCB.06763-11. Epub 2012 Mar 26.


The ubiquitin ligase Rsp5 is required for ribosome stability in Saccharomyces cerevisiae.

Shcherbik N, Pestov DG.

RNA. 2011 Aug;17(8):1422-8. doi: 10.1261/rna.2615311. Epub 2011 Jun 10.


5'-end surveillance by Xrn2 acts as a shared mechanism for mammalian pre-rRNA maturation and decay.

Wang M, Pestov DG.

Nucleic Acids Res. 2011 Mar;39(5):1811-22. doi: 10.1093/nar/gkq1050. Epub 2010 Oct 29.


Mammalian DEAD box protein Ddx51 acts in 3' end maturation of 28S rRNA by promoting the release of U8 snoRNA.

Srivastava L, Lapik YR, Wang M, Pestov DG.

Mol Cell Biol. 2010 Jun;30(12):2947-56. doi: 10.1128/MCB.00226-10. Epub 2010 Apr 19.


Polyadenylation and degradation of incomplete RNA polymerase I transcripts in mammalian cells.

Shcherbik N, Wang M, Lapik YR, Srivastava L, Pestov DG.

EMBO Rep. 2010 Feb;11(2):106-11. doi: 10.1038/embor.2009.271. Epub 2010 Jan 8.


The 5' external transcribed spacer in mouse ribosomal RNA contains two cleavage sites.

Kent T, Lapik YR, Pestov DG.

RNA. 2009 Jan;15(1):14-20. doi: 10.1261/rna.1384709. Epub 2008 Nov 24.


Assays for ribosomal RNA processing and ribosome assembly.

Pestov DG, Lapik YR, Lau LF.

Curr Protoc Cell Biol. 2008 Jun;Chapter 22:Unit 22.11. doi: 10.1002/0471143030.cb2211s39.


Restricting conformational flexibility of the switch II region creates a dominant-inhibitory phenotype in Obg GTPase Nog1.

Lapik YR, Misra JM, Lau LF, Pestov DG.

Mol Cell Biol. 2007 Nov;27(21):7735-44. Epub 2007 Sep 4.


The WD-repeat protein GRWD1: potential roles in myeloid differentiation and ribosome biogenesis.

Gratenstein K, Heggestad AD, Fortun J, Notterpek L, Pestov DG, Fletcher BS.

Genomics. 2005 Jun;85(6):762-73.


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