Format

Send to

Choose Destination
  • Showing results for a modified search because your search retrieved no results.
See comment in PubMed Commons below
Nat Commun. 2015 Jan 23;6:5936. doi: 10.1038/ncomms6936.

DNA sequencing using polymerase substrate-binding kinetics.

Author information

1
Protein Engineering, Illumina Inc., 5200 Research Place, San Diego, California 92122, USA.
2
Engineering, Illumina Inc., 5200 Research Place, San Diego, California 92122, USA.
3
Bioinformatics, Illumina Inc., 5200 Research Place, San Diego, California 92122, USA.

Abstract

Next-generation sequencing (NGS) has transformed genomic research by decreasing the cost of sequencing. However, whole-genome sequencing is still costly and complex for diagnostics purposes. In the clinical space, targeted sequencing has the advantage of allowing researchers to focus on specific genes of interest. Routine clinical use of targeted NGS mandates inexpensive instruments, fast turnaround time and an integrated and robust workflow. Here we demonstrate a version of the Sequencing by Synthesis (SBS) chemistry that potentially can become a preferred targeted sequencing method in the clinical space. This sequencing chemistry uses natural nucleotides and is based on real-time recording of the differential polymerase/DNA-binding kinetics in the presence of correct or mismatch nucleotides. This ensemble SBS chemistry has been implemented on an existing Illumina sequencing platform with integrated cluster amplification. We discuss the advantages of this sequencing chemistry for targeted sequencing as well as its limitations for other applications.

PMID:
25612848
PMCID:
PMC4354037
DOI:
10.1038/ncomms6936
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center