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Int J Mol Sci. 2010 Apr 26;11(4):1944-55. doi: 10.3390/ijms11041944.

Effects of aflatoxin B(1) and fumonisin B(1) on the viability and induction of apoptosis in rat primary hepatocytes.

Author information

1
Centro de P&D de Proteção Ambiental, Instituto Biológico, São Paulo, SP, Brazil.

Abstract

The present study evaluated the effect of aflatoxin B(1) (AFB(1)) and fumonisin B(1) (FB(1)) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB(1) and FB(1) used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner.

KEYWORDS:

aflatoxin B1; apoptosis; fumonisin B1; primary hepatocytes

PMID:
20480051
PMCID:
PMC2871147
DOI:
10.3390/ijms11041944
[Indexed for MEDLINE]
Free PMC Article

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