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J Biophotonics. 2016 Dec;9(11-12):1157-1166. doi: 10.1002/jbio.201600107. Epub 2016 Jun 20.

Exploring the effects of low-level laser therapy on fibroblasts and tumor cells following gamma radiation exposure.

Author information

1
Nuclear Technology Postgraduate Program, University of Sao Paulo, Av. Prof. Lineu Prestes, 2242, 05508-000, São Paulo, Brazil.
2
Laboratory of Optical Therapy (Center for Lasers and Applications/Nuclear and Energy Research Institute), Av. Prof. Lineu Prestes, 2242, 05508-000, Brazil.
3
Biochemistry and Biophysical Laboratory, Butantan Institute, Av. Vital Brazil, 1500, São Paulo, 05503-900, Brazil.
4
Medical School, University of Sao Paulo, Av. Dr. Arnaldo, 455, 01246-903, São Paulo, Brazil.

Abstract

Ionizing radiation (IR) induces DNA damage and low-level laser therapy (LLLT) has been investigated to prevent or repair detrimental outcomes resulting from IR exposure. Few in vitro studies, however, explore the biological mechanisms underlying those LLLT benefits. Thus, in this work, fibroblasts and tumor cells are submitted to IR with doses of 2.5 Gy and 10 Gy. After twenty-four-h, the cells are exposed to LLLT with fluences of 30 J cm-2 , 90 J cm-2 , and 150 J cm-2 . Cellular viability, cell cycle phases, cell proliferation index and senescence are evaluated on days 1 and 4 after LLLT irradiation. For fibroblasts, LLLT promotes - in a fluence-dependent manner - increments in cell viability and proliferation, while a reduction in the senescence was observed. Regarding tumor cells, no influences of LLLT on cell viability are noticed. Whereas LLLT enhances cell populations in S and G2 /M cell cycle phases for both cellular lines, a decrease in proliferation and increase in senescence was verified only for tumor cells. Putting together, the results suggest that fibroblasts and tumor cells present different responses to LLLT following exposure to gamma-radiation, and these promising results should stimulate further investigations. Senescence of tumor cells and fibroblasts on the 4th day after ionizing radiation (IR) and low-level laser therapy (LLLT) exposures. The number of senescent cells increased significantly for tumor cells (a) while for fibroblasts no increment was observed (b). The blue collor indicates senescence activity.

KEYWORDS:

breast cancer cells; fibroblasts; ionizing radiation; photobiomodulation; red laser

PMID:
27322660
DOI:
10.1002/jbio.201600107
[Indexed for MEDLINE]

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