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Viruses. 2018 Sep 11;10(9). pii: E486. doi: 10.3390/v10090486.

Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark.

Author information

1
DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. chmari@vet.dtu.dk.
2
DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. march@vet.dtu.dk.
3
The Natural History Museum of Denmark, University of Copenhagen, 2100 Copenhagen, Denmark. hjbaagoe@snm.ku.dk.
4
The Natural History Museum of Denmark, University of Copenhagen, 2100 Copenhagen, Denmark. esbenfjederholt@gmail.com.
5
DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. dengyu127@163.com.
6
School of Animal Science, Xichang College, Xichang 615013, China. dengyu127@163.com.
7
Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands. bart.kooi@wur.nl.
8
DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. grbe@vet.dtu.dk.
9
DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. aneb@vet.dtu.dk.
10
DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. tbrur@vet.dtu.dk.

Abstract

Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.

KEYWORDS:

Europe; Vespertilionidae; coronavirus; host restriction; nucleotide sequencing; phylogenetic analysis

PMID:
30208582
PMCID:
PMC6163574
DOI:
10.3390/v10090486
[Indexed for MEDLINE]
Free PMC Article

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