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BMC Complement Altern Med. 2015 Oct 5;15:345. doi: 10.1186/s12906-015-0876-0.

The anti-aging properties of a human placental hydrolysate combined with dieckol isolated from Ecklonia cava.

Author information

1
Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon, 210-702, Republic of Korea.
2
College of Pharmacy, Chung-Ang University, Heuksuk-dong, Dongjak-gu, Seoul, 156-756, Republic of Korea.
3
Industry-Academic Cooperation Foundation, Hanbat National University, Daejeon, 305-719, Republic of Korea.
4
Central Research Institute, Dr. Chung's Food Co. Ltd., Chungbuk, 361-782, Republic of Korea.
5
Chungbuk National University, Chungbuk, 361-763, Republic of Korea.
6
DF-Dr. Han Biotech., Shaoyaojubeili, Chaoyang District, Beijing, 10029, China.
7
Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon, 210-702, Republic of Korea. ssj66@gwnu.ac.kr.

Abstract

BACKGROUNDS:

In the present study, we aimed to examine the anti-aging properties of human placental hydrolysate (HPE) and dieckol (DE) from Ecklonia cava against free radical scavenging, muscle hypertrophy-related follistatin mRNA expression, amelioration of cognition-related genes and proteins, inhibition of collagenase-regulating genes, and elastinase activity.

METHODS:

The anti-aging effects were examined in human fibroblast (CCD986sk), mouse myoblast (C2C12), and neuroblastoma (N2a) cell models, by employing various assays such as 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) scavenging, hydroxyl radical-mediated oxidation, quantitative real-time polymerase chain reaction, enzyme activity, and immunocytochemistry observation.

RESULTS:

Our results show that HPE combined with DE (HPE:DE) strongly scavenged DPPH radicals and protected proteins against degradation by hydroxyl radical attack. HPE:DE effectively inhibited matrix metalloproteinase-1 expression, protein kinase C alpha expression, and elastinase activity. Furthermore, HPE:DE improved the expression of cognition-related genes (choline acetyltransferase and vesicular acetylcholine transporter). These events may proactively contribute to retard the aging processes and the abrupt physiological changes probably induced by mitochondrial dysfunction with aging.

CONCLUSIONS:

Based on these findings, we conclude that the combined treatment of HPE:DE may be useful for anti-aging therapy in which the accumulation of oxidative damage is the main driving force.

PMID:
26438076
PMCID:
PMC4594884
DOI:
10.1186/s12906-015-0876-0
[Indexed for MEDLINE]
Free PMC Article

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