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Macromol Rapid Commun. 2016 Mar;37(5):439-45. doi: 10.1002/marc.201500590. Epub 2015 Dec 22.

Transferrin Decorated Thermoresponsive Nanogels as Magnetic Trap Devices for Circulating Tumor Cells.

Author information

1
Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany.
2
LAMAP Laboratorio de Materiales Poliméricos, IMBIV-CONICET, Departamento de Química, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, X5000HUA, Córdoba, Argentina.
3
MAEER's Maharashtra Institute of Pharmacy, Kothrud, Pune, 411038, Maharashtra, India.
4
Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, D-14109, Berlin, Germany.

Abstract

A rational design of magnetic capturing nanodevices, based on a specific interaction with circulating tumor cells (CTCs), can advance the capturing efficiency and initiate the development of modern smart nanoformulations for rapid isolation and detection of these CTCs from the bloodstream. Therefore, the development and evaluation of magnetic nanogels (MNGs) based on magnetic nanoparticles and linear thermoresponsive polyglycerol for the capturing of CTCs with overexpressed transferrin (Tf(+) ) receptors has been presented in this study. The MNGs are synthesized using a strain-promoted "click" approach which has allowed the in situ surface decoration with Tf-polyethylene glycol (PEG) ligands of three different PEG chain lengths as targeting ligands. An optimal value of around 30% of cells captures is achieved with a linker of eight ethylene glycol units. This study shows the potential of MNGs for the capture of CTCs and the necessity of precise control over the linkage of the targeting moiety to the capturing device.

KEYWORDS:

cell sorting; circulating tumor cells; thermoresponsive nanogels; transferrin

PMID:
26691543
DOI:
10.1002/marc.201500590
[Indexed for MEDLINE]

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