Format

Send to

Choose Destination
Polymers (Basel). 2018 Aug 18;10(8). pii: E923. doi: 10.3390/polym10080923.

Synthesis and Properties of Silk Fibroin/Konjac Glucomannan Blend Beads.

Author information

1
Faculdade de Engenharia Química, Universidade Estadual de Campinas, Campinas 13083-852, São Paulo, Brazil. carlagfranca@gmail.com.
2
Faculdade de Engenharia Química, Universidade Estadual de Campinas, Campinas 13083-852, São Paulo, Brazil. vicente.qmc@gmail.com.
3
Departamento de Ciencias Matemáticas y Físicas, Facultad de Ingeniería, Universidad Católica de Temuco, Temuco 4813302, Chile. jacobo.hernandez@uct.cl.
4
Núcleo de Investigación en Bioproductos y Materiales Avanzados (BioMa), Facultad de Ingeniería, Universidad Católica de Temuco, Temuco 4781312, Chile. jacobo.hernandez@uct.cl.
5
Laboratório de Biotecnologia, Instituto de Biologia, Universidade Estadual de Campinas, Campinas 13083-862, São Paulo, Brazil. daisy.machado@gmail.com.
6
Laboratório de Biotecnologia, Instituto de Biologia, Universidade Estadual de Campinas, Campinas 13083-862, São Paulo, Brazil. mlancell@unicamp.br.
7
Faculdade de Engenharia Química, Universidade Estadual de Campinas, Campinas 13083-852, São Paulo, Brazil. beppu@feq.unicamp.br.

Abstract

Silk fibroin (SF) and konjac glucomannan (KGM) are promising materials in the biomedical field due to their low toxicity, biocompatibility, biodegradability and low immune response. Beads of these natural polymers are interesting scaffolds for biomedical applications, but their fabrication is a challenge due to their low stability and the necessary adaptation of their chemical and mechanical properties to be successfully applied. In that sense, this study aimed to synthesize a blend of silk fibroin and konjac glucomannan (SF/KGM) in the form of porous beads obtained through dripping into liquid nitrogen, with a post-treatment using ethanol. Intermolecular hydrogen bonds promoted the integration of SF and KGM. Treated beads showed higher porous size, crystallinity, and stability than untreated beads. Characterization analyses by Fourier-transform infrared spectroscopy (FTIR), thermogravimetric (TGA), and X-ray diffraction (XDR) evidenced that ethanol treatment allows a conformational transition from silk I to silk II in SF and an increase in the KGM deacetylation. Those chemical changes significantly enhanced the mechanical resistance of SF/KGM beads in comparison to pure SF and KGM beads. Moreover, samples showed cytocompatibility with HaCaT and BALB/c 3T3 cells.

KEYWORDS:

biopolymers; konjac glucomannan; porous beads; scaffolds; silk fibroin; tissue engineering

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center