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Viruses. 2019 Feb 22;11(2). pii: E188. doi: 10.3390/v11020188.

Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System.

Author information

1
Department of Biological Sciences, Universidad de Los Andes, 111711 Bogotá, Colombia. angela.holguin02@gmail.com.
2
Department of Biological Sciences, Universidad de Los Andes, 111711 Bogotá, Colombia. p.cardenas10@uniandes.edu.co.
3
Department of Biological Sciences, Universidad de Los Andes, 111711 Bogotá, Colombia. cat-prad@uniandes.edu.co.
4
Instituto René Rachou, Fundação Oswaldo Cruz, 21040-900 Belo Horizonte, Brazil. rabelo.leite@gmail.com.
5
Department of Biological Sciences, Universidad de Los Andes, 111711 Bogotá, Colombia. mc.buitrago10@uniandes.edu.co.
6
Department of Biological Sciences, Universidad de Los Andes, 111711 Bogotá, Colombia. in-clavi@uniandes.edu.co.
7
Instituto René Rachou, Fundação Oswaldo Cruz, 21040-900 Belo Horizonte, Brazil. guilherme.oliveira@itv.org.
8
Instituto Tecnológico Vale, 66055-090 Belém, Brazil. guilherme.oliveira@itv.org.
9
Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. pile@food.dtu.dk.
10
Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. fmaa@food.dtu.dk.
11
Department of Biological Sciences, Universidad de Los Andes, 111711 Bogotá, Colombia. mvives@uniandes.edu.co.

Abstract

Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and φSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy.

KEYWORDS:

Salmonella Enteritidis; antibiotics; bacteria-phage coevolution; bacterial resistance; phage-therapy

PMID:
30813274
PMCID:
PMC6410252
DOI:
10.3390/v11020188
[Indexed for MEDLINE]
Free PMC Article

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