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Microorganisms. 2019 Mar 5;7(3). pii: E71. doi: 10.3390/microorganisms7030071.

Bacillus subtilis DSM 32315 Supplementation Attenuates the Effects of Clostridium perfringens Challenge on the Growth Performance and Intestinal Microbiota of Broiler Chickens.

Author information

1
Department of Poultry Science, University of Georgia, Athens, GA 30602, USA. bortoluzzi.c@gmail.com.
2
Department of Poultry Science, University of Georgia, Athens, GA 30602, USA. vieirabs@hotmail.com.
3
Evonik Nutrition & Care GmbH, 63457 Hanau, Germany. juliano.dorigam@evonik.com.
4
Evonik Corporation, Kennesaw, GA 30144, USA. anita.menconi@evonik.com.
5
Evonik Corporation, Kennesaw, GA 30144, USA. adebayo.sokale@evonik.com.
6
Evonik Nutrition & Care GmbH, 63457 Hanau, Germany. kiran.doranalli@evonik.com.
7
Department of Poultry Science, University of Georgia, Athens, GA 30602, USA. applegt@uga.edu.

Abstract

The objective of this study was to evaluate the effects of the dietary supplementation of Bacillus subtilis DSM 32315 (probiotic) on the performance and intestinal microbiota of broiler chickens infected with Clostridium perfringens (CP). One-day-old broiler chickens were assigned to 3 treatments with 8 replicate pens (50 birds/pen). The treatments were: non-infected control; infected control; and infected supplemented with probiotic (1 × 10⁶ CFU/g of feed). On day of hatch, all birds were sprayed with a coccidia vaccine based on the manufacturer recommended dosage. On d 18⁻20 the infected birds were inoculated with CP via feed. Necrotic enteritis (NE) lesion score was performed on d 21. Digestive tract of 2 birds/pen was collected on d 21 to analyze the ileal and cecal microbiota by 16S rRNA sequencing. Performance was evaluated on d 28 and 42. On d 21, probiotic supplementation reduced (p < 0.001) the severity of NE related lesion versus infected control birds. On d 28, feed efficiency was improved (p < 0.001) in birds supplemented with probiotic versus infected control birds. On d 42, body weight gain (BW gain) and feed conversion ratio (FCR) were improved (p < 0.001) in probiotic supplemented birds versus infected control birds. The diversity, composition and predictive function of the intestinal microbial digesta changed with the infection but the supplementation of probiotic reduced these variations. Therefore, dietary supplementation of Bacillus subtilis DSM 32315 was beneficial in attenuating the negative effects of CP challenge on the performance and intestinal microbiota of broilers chickens.

KEYWORDS:

Bacillus subtilis; broiler; microbiota; necrotic enteritis

Conflict of interest statement

The authors declare no conflict of interest. The study was financially funded by Evonik Nutrition & Care GmbH.

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