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Oncol Lett. 2018 Oct;16(4):4543-4550. doi: 10.3892/ol.2018.9170. Epub 2018 Jul 18.

miRNA-199a-5p suppresses proliferation and invasion by directly targeting NF-κB1 in human ovarian cancer cells.

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Department of Internal Medicine-Oncology, Xinchang People's Hospital of Zhejiang, Shaoxing, Zhejiang 312500, P.R. China.
Department of Intensive Care Unit, Putuo Hospital of Zhejiang, Zhoushan, Zhejiang 316100, P.R. China.
Department of General Surgery, Shaoxing Hospital of China Medical University, Shaoxing, Zhejiang 312030, P.R. China.


The aberrant expression of microRNA (miRNA)-199a-5p has been frequently reported in a number of cancer types, but to the best of our knowledge, this has not been reported in ovarian cancer (OC). The role and the molecular mechanism of miR-199a-5p in OC have not been reported. Therefore, the present study investigated the effects of miR-199a-5p overexpression on the proliferation and invasion of OC cells. The level of miR-199a-5p in OC cell lines was determined by reverse transcription-quantitative polymerase chain reaction. The miR-199a-5p mimic was transiently transfected into OC cells using Lipofectamine 2000 reagent. Subsequently, the BrdU-ELISA results indicated that the exogenous expression of miR-199a-5p inhibited cell proliferation. In addition, miR-199a-5p overexpression was able to inhibit the invasion of HO-8910 and ES-2 cells. RT-qPCR was performed to determine the expression of matrix metalloproteinase (MMP)-2 and -9 in OC cells. NF-κB1 expression was reduced by upregulation of miR-199a-5p. Bioinformatics analysis predicted that NF-κB1 was a potential target of miR-199a-5p. Luciferase reporter assay further confirmed that miR-199a-5p was able to directly target the 3'UTR of NF-κB1. In conclusion, miRNA-199a-5p may suppress the proliferation and invasion of human ovarian cancer cells by directly targeting NF-κB1.


invasion; microRNA-199a-5p; ovarian cancer; proliferation

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