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Oncol Lett. 2018 Oct;16(4):4543-4550. doi: 10.3892/ol.2018.9170. Epub 2018 Jul 18.

miRNA-199a-5p suppresses proliferation and invasion by directly targeting NF-κB1 in human ovarian cancer cells.

Author information

1
Department of Internal Medicine-Oncology, Xinchang People's Hospital of Zhejiang, Shaoxing, Zhejiang 312500, P.R. China.
2
Department of Intensive Care Unit, Putuo Hospital of Zhejiang, Zhoushan, Zhejiang 316100, P.R. China.
3
Department of General Surgery, Shaoxing Hospital of China Medical University, Shaoxing, Zhejiang 312030, P.R. China.

Abstract

The aberrant expression of microRNA (miRNA)-199a-5p has been frequently reported in a number of cancer types, but to the best of our knowledge, this has not been reported in ovarian cancer (OC). The role and the molecular mechanism of miR-199a-5p in OC have not been reported. Therefore, the present study investigated the effects of miR-199a-5p overexpression on the proliferation and invasion of OC cells. The level of miR-199a-5p in OC cell lines was determined by reverse transcription-quantitative polymerase chain reaction. The miR-199a-5p mimic was transiently transfected into OC cells using Lipofectamine 2000 reagent. Subsequently, the BrdU-ELISA results indicated that the exogenous expression of miR-199a-5p inhibited cell proliferation. In addition, miR-199a-5p overexpression was able to inhibit the invasion of HO-8910 and ES-2 cells. RT-qPCR was performed to determine the expression of matrix metalloproteinase (MMP)-2 and -9 in OC cells. NF-κB1 expression was reduced by upregulation of miR-199a-5p. Bioinformatics analysis predicted that NF-κB1 was a potential target of miR-199a-5p. Luciferase reporter assay further confirmed that miR-199a-5p was able to directly target the 3'UTR of NF-κB1. In conclusion, miRNA-199a-5p may suppress the proliferation and invasion of human ovarian cancer cells by directly targeting NF-κB1.

KEYWORDS:

invasion; microRNA-199a-5p; ovarian cancer; proliferation

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