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Front Microbiol. 2016 Mar 2;7:242. doi: 10.3389/fmicb.2016.00242. eCollection 2016.

Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate.

Author information

1
Tianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Peking Union Medical College - Chinese Academy of Medical Sciences Tianjin, China.
2
Department of Materials Chemistry, Nankai University Tianjin, China.
3
Tianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Peking Union Medical College - Chinese Academy of Medical SciencesTianjin, China; Tianjin Medical University Cancer Institute and HospitalTianjin, China.
4
Tianjin Medical University Cancer Institute and Hospital Tianjin, China.

Abstract

Lysine-porphyrin conjugate 4i has potent photosensitive antibacterial effect on clinical isolated bacterial strains such as Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa. The mechanism of photodynamic antibacterial chemotherapy of 4i (4i-PACT) in vitro and the treatment effect in vivo was investigated in this paper. Atomic force microscopy (AFM) revealed that 4i-PACT can effectively destroy membrane and wall of bacteria, resulting in leakage of its content. This was confirmed by dual fluorescent staining with acridine orange/ethidium bromide and measuring materials absorption at 260 nm. Agarose gel electrophoresis measurement showed that 4i-PACT can damage genomic DNA. Healing of wound in rat infected by mixed bacteria showed that the efficiency of 4i-PACT is dependent on the dose of light. These results showed that 4i-PACT has promising bactericidal effect both in vitro and in vivo.

KEYWORDS:

antibiotic resistance; lysine-porphyrin conjugate; mixed bacterial-infected wounds; photodynamic antimicrobial chemotherapy; photosensitizer

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