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Mar Drugs. 2017 Jun 22;15(7). pii: E193. doi: 10.3390/md15070193.

Fucoidan Does Not Exert Anti-Tumorigenic Effects on Uveal Melanoma Cell Lines.

Author information

1
Department of Ophthalmology, University of Kiel, University Medical Center, Arnold-Heller-Str. 3, 24105 Kiel, Germany. michaela_dithmer@web.de.
2
Department of Ophthalmology, University of Kiel, University Medical Center, Arnold-Heller-Str. 3, 24105 Kiel, Germany. anna.maria.kirsch@gmx.de.
3
Department of Ophthalmology, University of Kiel, University Medical Center, Arnold-Heller-Str. 3, 24105 Kiel, Germany. elisabeth.richert@uksh.de.
4
Experimental Trauma Surgery, University of Kiel, University Medical Center, Arnold-Heller-Str. 3, 24105 Kiel, Germany. sabine.fuchs@uksh.de.
5
Experimental Trauma Surgery, University of Kiel, University Medical Center, Arnold-Heller-Str. 3, 24105 Kiel, Germany. fanlu.wang@uksh.de.
6
MetaPhysiol, Am Römerberg, 55270 Essenheim, Germany. schmidt@metaphysiol.de.
7
Department of Molecular and Clinical Cancer Medicine, Liverpool Ocular Oncology Research Group, Pathology, University of Liverpool, Liverpool L69 3BX, UK. s.e.coupland@liverpool.ac.uk.
8
Department of Ophthalmology, University of Kiel, University Medical Center, Arnold-Heller-Str. 3, 24105 Kiel, Germany. johann.roider@uksh.de.
9
Department of Ophthalmology, University of Kiel, University Medical Center, Arnold-Heller-Str. 3, 24105 Kiel, Germany. aklettner@auge.uni-kiel.de.

Abstract

BACKGROUND:

The polysaccharide fucoidan is widely investigated as an anti-cancer agent. Here, we tested the effect of fucoidan on uveal melanoma cell lines.

METHODS:

The effect of 100 µM fucoidan was investigated on five cell lines (92.1, Mel270 OMM1, OMM2.3, OMM2.5) and of 1 µg/mL-1 mg/mL fucoidan in two cell lines (OMM1, OMM2.3). Cell proliferation and viability were investigated with a WST-1 assay, migration in a wound healing (scratch) assay. Vascular Endothelial Growth Factor (VEGF) was measured in ELISA. Angiogenesis was evaluated in co-cultures with endothelial cells. Cell toxicity was induced by hydrogen-peroxide. Protein expression (Akt, ERK1/2, Bcl-2, Bax) was investigated in Western blot.

RESULTS:

Fucoidan increased proliferation in two and reduced it in one cell line. Migration was reduced in three cell lines. The effect of fucoidan on VEGF was cell type and concentration dependent. In endothelial co-culture with 92.1, fucoidan significantly increased tubular structures. Moreover, fucoidan significantly protected all tested uveal melanoma cell lines from hydrogen-peroxide induced cell death. Under oxidative stress, fucoidan did not alter the expression of Bcl-2, Bax or ERK1/2, while inducing Akt expression in 92.1 cells but not in any other cell line.

CONCLUSION:

Fucoidan did not show anti-tumorigenic effects but displayed protective and pro-angiogenic properties, rendering fucoidan unsuitable as a potential new drug for the treatment of uveal melanoma.

KEYWORDS:

VEGF; angiogenesis; fucoidan; oxidative stress; uveal melanoma

PMID:
28640204
PMCID:
PMC5532635
DOI:
10.3390/md15070193
[Indexed for MEDLINE]
Free PMC Article

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