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Items: 1 to 20 of 51

1.

Finally, Archaea Get Their CRISPR-Cas Toolbox.

Gophna U, Allers T, Marchfelder A.

Trends Microbiol. 2017 Jun;25(6):430-432. doi: 10.1016/j.tim.2017.03.009. Epub 2017 Apr 6.

PMID:
28391963
2.

The crystal structure of Trz1, the long form RNase Z from yeast.

Ma M, Li de la Sierra-Gallay I, Lazar N, Pellegrini O, Durand D, Marchfelder A, Condon C, van Tilbeurgh H.

Nucleic Acids Res. 2017 Jun 2;45(10):6209-6216. doi: 10.1093/nar/gkx216.

3.

High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon.

Stachler AE, Turgeman-Grott I, Shtifman-Segal E, Allers T, Marchfelder A, Gophna U.

Nucleic Acids Res. 2017 May 19;45(9):5208-5216. doi: 10.1093/nar/gkx150.

4.

Genome-wide identification of transcriptional start sites in the haloarchaeon Haloferax volcanii based on differential RNA-Seq (dRNA-Seq).

Babski J, Haas KA, Näther-Schindler D, Pfeiffer F, Förstner KU, Hammelmann M, Hilker R, Becker A, Sharma CM, Marchfelder A, Soppa J.

BMC Genomics. 2016 Aug 12;17(1):629. doi: 10.1186/s12864-016-2920-y.

5.

Gene Repression in Haloarchaea Using the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas I-B System.

Stachler AE, Marchfelder A.

J Biol Chem. 2016 Jul 15;291(29):15226-42. doi: 10.1074/jbc.M116.724062. Epub 2016 May 16.

6.

The role of Cas8 in type I CRISPR interference.

Cass SD, Haas KA, Stoll B, Alkhnbashi OS, Sharma K, Urlaub H, Backofen R, Marchfelder A, Bolt EL.

Biosci Rep. 2015 May 5;35(3). pii: e00197. doi: 10.1042/BSR20150043.

7.

Analysis of protein-RNA interactions in CRISPR proteins and effector complexes by UV-induced cross-linking and mass spectrometry.

Sharma K, Hrle A, Kramer K, Sachsenberg T, Staals RH, Randau L, Marchfelder A, van der Oost J, Kohlbacher O, Conti E, Urlaub H.

Methods. 2015 Nov 1;89:138-48. doi: 10.1016/j.ymeth.2015.06.005. Epub 2015 Jun 10.

PMID:
26071038
8.

Deletion of the Sm1 encoding motif in the lsm gene results in distinct changes in the transcriptome and enhanced swarming activity of Haloferax cells.

Maier LK, Benz J, Fischer S, Alstetter M, Jaschinski K, Hilker R, Becker A, Allers T, Soppa J, Marchfelder A.

Biochimie. 2015 Oct;117:129-37. doi: 10.1016/j.biochi.2015.02.023. Epub 2015 Mar 6.

9.

The Adaptive Immune System of Haloferax volcanii.

Maier LK, Dyall-Smith M, Marchfelder A.

Life (Basel). 2015 Feb 16;5(1):521-37. doi: 10.3390/life5010521. Review.

10.

An active immune defense with a minimal CRISPR (clustered regularly interspaced short palindromic repeats) RNA and without the Cas6 protein.

Maier LK, Stachler AE, Saunders SJ, Backofen R, Marchfelder A.

J Biol Chem. 2015 Feb 13;290(7):4192-201. doi: 10.1074/jbc.M114.617506. Epub 2014 Dec 15.

11.

Structural analyses of the CRISPR protein Csc2 reveal the RNA-binding interface of the type I-D Cas7 family.

Hrle A, Maier LK, Sharma K, Ebert J, Basquin C, Urlaub H, Marchfelder A, Conti E.

RNA Biol. 2014;11(8):1072-82. doi: 10.4161/rna.29893.

12.

Small regulatory RNAs in Archaea.

Babski J, Maier LK, Heyer R, Jaschinski K, Prasse D, Jäger D, Randau L, Schmitz RA, Marchfelder A, Soppa J.

RNA Biol. 2014;11(5):484-93. Epub 2014 Mar 31. Review.

13.

Generation and phenotyping of a collection of sRNA gene deletion mutants of the haloarchaeon Haloferax volcanii.

Jaschinski K, Babski J, Lehr M, Burmester A, Benz J, Heyer R, Dörr M, Marchfelder A, Soppa J.

PLoS One. 2014 Mar 17;9(3):e90763. doi: 10.1371/journal.pone.0090763. eCollection 2014.

14.

A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (crispr)-derived rnas (crrnas) in Haloferax volcanii.

Brendel J, Stoll B, Lange SJ, Sharma K, Lenz C, Stachler AE, Maier LK, Richter H, Nickel L, Schmitz RA, Randau L, Allers T, Urlaub H, Backofen R, Marchfelder A.

J Biol Chem. 2014 Mar 7;289(10):7164-77. doi: 10.1074/jbc.M113.508184. Epub 2014 Jan 23.

15.

Requirements for a successful defence reaction by the CRISPR-Cas subtype I-B system.

Stoll B, Maier LK, Lange SJ, Brendel J, Fischer S, Backofen R, Marchfelder A.

Biochem Soc Trans. 2013 Dec;41(6):1444-8. doi: 10.1042/BST20130098. Review.

PMID:
24256235
16.

tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6.

Skowronek E, Grzechnik P, Späth B, Marchfelder A, Kufel J.

RNA. 2014 Jan;20(1):115-30. doi: 10.1261/rna.041467.113. Epub 2013 Nov 18.

17.

RNA-Pareto: interactive analysis of Pareto-optimal RNA sequence-structure alignments.

Schnattinger T, Schöning U, Marchfelder A, Kestler HA.

Bioinformatics. 2013 Dec 1;29(23):3102-4. doi: 10.1093/bioinformatics/btt536. Epub 2013 Sep 16.

PMID:
24045774
18.

Special focus CRISPR-Cas.

Marchfelder A.

RNA Biol. 2013 May;10(5):655-8. doi: 10.4161/rna.24687. No abstract available.

19.

Essential requirements for the detection and degradation of invaders by the Haloferax volcanii CRISPR/Cas system I-B.

Maier LK, Lange SJ, Stoll B, Haas KA, Fischer S, Fischer E, Duchardt-Ferner E, Wöhnert J, Backofen R, Marchfelder A.

RNA Biol. 2013 May;10(5):865-74. doi: 10.4161/rna.24282. Epub 2013 Apr 17.

20.

The immune system of halophilic archaea.

Maier LK, Fischer S, Stoll B, Brendel J, Pfeiffer F, Dyall-Smith M, Marchfelder A.

Mob Genet Elements. 2012 Sep 1;2(5):228-232.

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