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Items: 1 to 20 of 60


The Genome Sequence of the Halobacterium salinarum Type Strain Is Closely Related to That of Laboratory Strains NRC-1 and R1.

Pfeiffer F, Marchfelder A, Habermann B, Dyall-Smith ML.

Microbiol Resour Announc. 2019 Jul 11;8(28). pii: e00429-19. doi: 10.1128/MRA.00429-19.


Homologs of aquifex aeolicus protein-only RNase P are not the major RNase P activities in the archaea haloferax volcanii and methanosarcina mazei.

Schwarz TS, Wäber NB, Feyh R, Weidenbach K, Schmitz RA, Marchfelder A, Hartmann RK.

IUBMB Life. 2019 Aug;71(8):1109-1116. doi: 10.1002/iub.2122. Epub 2019 Jul 8.


Solution structure and dynamics of the small protein HVO_2922 from Haloferax volcanii.

Kubatova N, Jonker HRA, Saxena K, Richter C, Vogel V, Schreiber S, Marchfelder A, Schwalbe H.

Chembiochem. 2019 Jun 4. doi: 10.1002/cbic.201900085. [Epub ahead of print]


CRISPRi as an efficient tool for gene repression in archaea.

Stachler AE, Schwarz TS, Schreiber S, Marchfelder A.

Methods. 2019 May 28. pii: S1046-2023(18)30472-9. doi: 10.1016/j.ymeth.2019.05.023. [Epub ahead of print] Review.


CRISPR-Cas: more than ten years and still full of mysteries.

Charpentier E, Elsholz A, Marchfelder A.

RNA Biol. 2019 Apr;16(4):377-379. doi: 10.1080/15476286.2019.1591659. No abstract available.


It's all about the T: transcription termination in archaea.

Maier LK, Marchfelder A.

Biochem Soc Trans. 2019 Feb 28;47(1):461-468. doi: 10.1042/BST20180557. Epub 2019 Feb 19. Review.


Pervasive acquisition of CRISPR memory driven by inter-species mating of archaea can limit gene transfer and influence speciation.

Turgeman-Grott I, Joseph S, Marton S, Eizenshtein K, Naor A, Soucy SM, Stachler AE, Shalev Y, Zarkor M, Reshef L, Altman-Price N, Marchfelder A, Gophna U.

Nat Microbiol. 2019 Jan;4(1):177-186. doi: 10.1038/s41564-018-0302-8. Epub 2018 Nov 26.


Insights into RNA-processing pathways and associated RNA-degrading enzymes in Archaea.

Clouet-d'Orval B, Batista M, Bouvier M, Quentin Y, Fichant G, Marchfelder A, Maier LK.

FEMS Microbiol Rev. 2018 Sep 1;42(5):579-613. doi: 10.1093/femsre/fuy016. Review.


The nuts and bolts of the Haloferax CRISPR-Cas system I-B.

Maier LK, Stachler AE, Brendel J, Stoll B, Fischer S, Haas KA, Schwarz TS, Alkhnbashi OS, Sharma K, Urlaub H, Backofen R, Gophna U, Marchfelder A.

RNA Biol. 2019 Apr;16(4):469-480. doi: 10.1080/15476286.2018.1460994. Epub 2018 May 21.


Finally, Archaea Get Their CRISPR-Cas Toolbox.

Gophna U, Allers T, Marchfelder A.

Trends Microbiol. 2017 Jun;25(6):430-432. doi: 10.1016/j.tim.2017.03.009. Epub 2017 Apr 6.


The crystal structure of Trz1, the long form RNase Z from yeast.

Ma M, Li de la Sierra-Gallay I, Lazar N, Pellegrini O, Durand D, Marchfelder A, Condon C, van Tilbeurgh H.

Nucleic Acids Res. 2017 Jun 2;45(10):6209-6216. doi: 10.1093/nar/gkx216.


High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon.

Stachler AE, Turgeman-Grott I, Shtifman-Segal E, Allers T, Marchfelder A, Gophna U.

Nucleic Acids Res. 2017 May 19;45(9):5208-5216. doi: 10.1093/nar/gkx150.


Genome-wide identification of transcriptional start sites in the haloarchaeon Haloferax volcanii based on differential RNA-Seq (dRNA-Seq).

Babski J, Haas KA, Näther-Schindler D, Pfeiffer F, Förstner KU, Hammelmann M, Hilker R, Becker A, Sharma CM, Marchfelder A, Soppa J.

BMC Genomics. 2016 Aug 12;17(1):629. doi: 10.1186/s12864-016-2920-y.


Gene Repression in Haloarchaea Using the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas I-B System.

Stachler AE, Marchfelder A.

J Biol Chem. 2016 Jul 15;291(29):15226-42. doi: 10.1074/jbc.M116.724062. Epub 2016 May 16.


The role of Cas8 in type I CRISPR interference.

Cass SD, Haas KA, Stoll B, Alkhnbashi OS, Sharma K, Urlaub H, Backofen R, Marchfelder A, Bolt EL.

Biosci Rep. 2015 May 5;35(3). pii: e00197. doi: 10.1042/BSR20150043.


Analysis of protein-RNA interactions in CRISPR proteins and effector complexes by UV-induced cross-linking and mass spectrometry.

Sharma K, Hrle A, Kramer K, Sachsenberg T, Staals RH, Randau L, Marchfelder A, van der Oost J, Kohlbacher O, Conti E, Urlaub H.

Methods. 2015 Nov 1;89:138-48. doi: 10.1016/j.ymeth.2015.06.005. Epub 2015 Jun 10.


Deletion of the Sm1 encoding motif in the lsm gene results in distinct changes in the transcriptome and enhanced swarming activity of Haloferax cells.

Maier LK, Benz J, Fischer S, Alstetter M, Jaschinski K, Hilker R, Becker A, Allers T, Soppa J, Marchfelder A.

Biochimie. 2015 Oct;117:129-37. doi: 10.1016/j.biochi.2015.02.023. Epub 2015 Mar 6.


The Adaptive Immune System of Haloferax volcanii.

Maier LK, Dyall-Smith M, Marchfelder A.

Life (Basel). 2015 Feb 16;5(1):521-37. doi: 10.3390/life5010521. Review.


An active immune defense with a minimal CRISPR (clustered regularly interspaced short palindromic repeats) RNA and without the Cas6 protein.

Maier LK, Stachler AE, Saunders SJ, Backofen R, Marchfelder A.

J Biol Chem. 2015 Feb 13;290(7):4192-201. doi: 10.1074/jbc.M114.617506. Epub 2014 Dec 15.


Structural analyses of the CRISPR protein Csc2 reveal the RNA-binding interface of the type I-D Cas7 family.

Hrle A, Maier LK, Sharma K, Ebert J, Basquin C, Urlaub H, Marchfelder A, Conti E.

RNA Biol. 2014;11(8):1072-82. doi: 10.4161/rna.29893.

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