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Molecules. 2018 May 25;23(6). pii: E1259. doi: 10.3390/molecules23061259.

Hydrophobic Amino Acid Content in Onions as Potential Fingerprints of Geographical Origin: The Case of Rossa da Inverno sel. Rojo Duro.

Author information

1
Department of Pharmaceutical Sciences, Section of Chemistry and Technology of Drugs, University of Perugia, Via del Liceo 1, 06123 Perugia, Italy. federica.ianni@chimfarm.unipg.it.
2
Department of Pharmaceutical Sciences, Section of Chemistry and Technology of Drugs, University of Perugia, Via del Liceo 1, 06123 Perugia, Italy. antonellalisanti86@gmail.com.
3
Department of Pharmaceutical Sciences, Section of Chemistry and Technology of Drugs, University of Perugia, Via del Liceo 1, 06123 Perugia, Italy. maura.marinozzi@unipg.it.
4
Department of Pharmaceutical Sciences, Section of Chemistry and Technology of Drugs, University of Perugia, Via del Liceo 1, 06123 Perugia, Italy. emidio.camaioni@unipg.it.
5
Department of Pharmaceutical Sciences, Section of Chemistry and Technology of Drugs, University of Perugia, Via del Liceo 1, 06123 Perugia, Italy. lucia.pucciarini@hotmail.it.
6
3A-Umbria Agrifood Technology Park, Fraz. Pantalla, 06059 Todi, Italy. reteagrometeo@parco3a.org.
7
Department of Pharmaceutical Sciences, Section of Chemistry and Technology of Drugs, University of Perugia, Via del Liceo 1, 06123 Perugia, Italy. roccaldo.sardella@unipg.it.
8
3A-Umbria Agrifood Technology Park, Fraz. Pantalla, 06059 Todi, Italy. lconcezzi@parco3a.org.
9
Department of Pharmaceutical Sciences, Section of Chemistry and Technology of Drugs, University of Perugia, Via del Liceo 1, 06123 Perugia, Italy. benedetto.natalini@unipg.it.

Abstract

In this study, we were interested in comparing the amino acid profile in a specific variety of onion, Rossa da inverno sel. Rojo Duro, produced in two different Italian sites: the Cannara (Umbria region) and Imola (Emilia Romagna region) sites. Onions were cultivated in a comparable manner, mostly in terms of the mineral fertilization, seeding, and harvesting stages, as well as good weed control. Furthermore, in both regions, the plants were irrigated by the water sprinkler method and subjected to similar temperature and weather conditions. A further group of Cannara onions that were grown by micro-irrigation was also evaluated. After the extraction of the free amino acid mixture, an ion-pairing reversed-phase (IP-RP) HPLC method allowed for the separation and the evaporative light scattering detection of almost all the standard proteinogenic amino acids. However, only the peaks corresponding to leucine (Leu), phenylalanine (Phe), and tryptophan (Trp), were present in all the investigated samples and they were unaffected from the matrix interfering peaks. The use of the beeswarm/box plots revealed that the content of Leu and Phe were markedly influenced by the geographical origin of the onions (with *** p.

KEYWORDS:

HPLC analysis; Rossa da inverno sel. Rojo Duro onion cultivar; amino acids content; food traceability; geographical origin; statistical evaluations

PMID:
29799448
PMCID:
PMC6099655
DOI:
10.3390/molecules23061259
[Indexed for MEDLINE]
Free PMC Article

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