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Electrophoresis. 2015 Jul;36(14):1564-71. doi: 10.1002/elps.201400576. Epub 2015 May 26.

Potential of prodendronic polyamines with modulated segmental charge density as novel coating for fast and efficient analysis of peptides and basic proteins by CE and CE-MS.

Author information

1
Laboratory of Foodomics, Institute of Food Science Research (CIAL), CSIC, Campus de Cantoblanco, Madrid, Spain.
2
CAPES Foundation, Ministry of Education of Brazil, Brasília, DF, Brazil.
3
Departamento de Química Física y Analítica, University of Jaen, Spain.
4
Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute, Facoltà di Farmacia, Università degli Studi di Messina, Messina, Italy.
5
Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Madrid.

Abstract

In this work, the suitability of a new polymer family has been investigated as capillary coatings for the analysis of peptides and basic proteins by CE. This polymer family has been designed to minimize or completely prevent protein-capillary wall interactions and to modify the EOF. These coating materials are linear polymeric chains bearing as side cationizable moiety a dentronic triamine derived from N,N,N',N'-tetraethyldiethylenetriamine (TEDETA), which is linked to the backbone through a spacer (unit labeled as TEDETAMA). Four different polymers have been prepared and evaluated: a homopolymer which comprised only of those cationizable repetitive units of TEDETAMA, and three copolymers that randomly incorporate TEDETAMA together with neutral hydrosoluble units of N-(2-hydroxypropyl) methacrylamide (HPMA) at different molar percentages (25:75, 50:50 and 75:25). It has been demonstrated that the composition of the copolymers influences the EOF and therefore the separation of the investigated biopolymers. Among the novel polymers studied, poly-(TEDETAMA-co-HPMA) 50:50 copolymer was successfully applied as coating material of the inner capillary surface in CE-UV and CE-MS, providing EOF reversing together with fast and efficient baseline separation of peptides and basic proteins. Finally, the feasibility of the polymer-coated capillary was shown through the analysis of lysozyme in a cheese sample.

KEYWORDS:

Basic proteins; CE-MS; N,N,N’,N’-tetraethyldiethylenetriamine; N-(2-hydroxy-propyl) methacrylamide; Peptides

PMID:
25820626
DOI:
10.1002/elps.201400576
[Indexed for MEDLINE]

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