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Nutrients. 2018 Sep 14;10(9). pii: E1304. doi: 10.3390/nu10091304.

Cardioprotective Effects of Nanoemulsions Loaded with Anti-Inflammatory Nutraceuticals against Doxorubicin-Induced Cardiotoxicity.

Author information

1
Division of Cardiology, Istituto Nazionale Tumori⁻IRCCS-Fondazione G.Pascale, 80131 Napoli, Italy. quagliariello.enzo@gmail.com.
2
Center for Advanced Biomaterial for Health Care (CABHC), Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Naples, Italy. Raffaele.Vecchione@iit.it.
3
Division of Cardiology, Istituto Nazionale Tumori⁻IRCCS-Fondazione G.Pascale, 80131 Napoli, Italy. melinacoppola@libero.it.
4
Center for Advanced Biomaterial for Health Care (CABHC), Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Naples, Italy. chiara.dicicco@iit.it.
5
Center for Advanced Biomaterial for Health Care (CABHC), Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Naples, Italy. alberta.decapua@iit.it.
6
Division of Cardiology, Istituto Nazionale Tumori⁻IRCCS-Fondazione G.Pascale, 80131 Napoli, Italy. gpiscopo84@gmail.com.
7
Division of Cardiology, Istituto Nazionale Tumori⁻IRCCS-Fondazione G.Pascale, 80131 Napoli, Italy. rolando.paciello@unina.it.
8
Department of Pharmacy, University of Naples Federico II, via D. Montesano 49, 80131 Naples, Italy. viviana.narciso@unina.it.
9
Department of Pharmacy, University of Naples Federico II, via D. Montesano 49, 80131 Naples, Italy. carmen.formisano2@unina.it.
10
Department of Pharmacy, University of Naples Federico II, via D. Montesano 49, 80131 Naples, Italy. scatagli@unina.it.
11
Department of Medical Oncology, Istituto Nazionale Tumori⁻IRCCS-Fondazione G.Pascale, 80131 Napoli, Italy. rv.iaffaioli@gmail.com.
12
Scientific Direction, Istituto Nazionale Tumori⁻IRCCS-Fondazione G.Pascale, 80131 Napoli, Italy. g.botti@istitutotumori.na.it.
13
Center for Advanced Biomaterial for Health Care (CABHC), Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Naples, Italy. Paolo.Netti@iit.it.
14
Division of Cardiology, Istituto Nazionale Tumori⁻IRCCS-Fondazione G.Pascale, 80131 Napoli, Italy. n.maurea@istitutotumori.na.it.

Abstract

Doxorubicin is a highly active antineoplastic agent, but its clinical use is limited because of its cardiotoxicity. Although nutraceuticals endowed with anti-inflammatory properties exert cardioprotective activity, their bioavailability and stability are inconsistent. In an attempt to address this issue, we evaluated whether bioavailable nanoemulsions loaded with nutraceuticals (curcumin and fresh and dry tomato extracts rich in lycopene) protect cardiomyoblasts (H9C2 cells) from doxorubicin-induced toxicity. Nanoemulsions were produced with a high-pressure homogenizer. H9C2 cells were incubated with nanoemulsions loaded with different nutraceuticals alone or in combination with doxorubicin. Cell viability was evaluated with a modified MTT method. The levels of the lipid peroxidation products malondialdehyde (MDA) and 4-hydroxy-2-butanone (4-HNA), and of the cardiotoxic-related interleukins IL-6, IL-8, IL-1β and IL-10, tumor necrosis factor-alpha (TNF-α), and nitric oxide were analyzed in cardiomyoblasts. The hydrodynamic size of nanoemulsions was around 100 nm. Cell viability enhancement was 35⁻40% higher in cardiomyoblasts treated with nanoemulsion + doxorubicin than in cardiomyoblasts treated with doxorubicin alone. Nanoemulsions also protected against oxidative stress as witnessed by a reduction of MDA and 4-HNA. Notably, nanoemulsions inhibited the release of IL-6, IL-8, IL-1β, TNF-α and nitric oxide by around 35⁻40% and increased IL-10 production by 25⁻27% versus cells not treated with emulsions. Of the nutraceuticals evaluated, lycopene-rich nanoemulsions had the best cardioprotective profile. In conclusion, nanoemulsions loaded with the nutraceuticals described herein protect against cardiotoxicity, by reducing inflammation and lipid oxidative stress. These results set the stage for studies in preclinical models.

KEYWORDS:

cardiology; cytokines; doxorubicin; nanomedicine; nutraceuticals

PMID:
30223482
PMCID:
PMC6164259
DOI:
10.3390/nu10091304
[Indexed for MEDLINE]
Free PMC Article

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