Format

Send to

Choose Destination
Appl Radiat Isot. 2011 Feb;69(2):373-6. doi: 10.1016/j.apradiso.2010.10.025. Epub 2010 Oct 29.

Study of DNA damage with a new system for irradiation of samples in a nuclear reactor.

Author information

1
Instituto Superior de Tecnologías y Ciencias Aplicadas, InSTEC; Avenida Salvador Allende y Luaces, Quinta de Los Molinos, Plaza de la Revolución, Havana, AP 6163, Cuba. mrgual@instec.cu

Abstract

In this paper, we report results of a quantitative analysis of the effects of neutrons on DNA, and, specifically, the production of simple and double breaks of plasmid DNA in aqueous solutions with different concentrations of free-radical scavengers. The radiation damage to DNA was evaluated by electrophoresis through agarose gels. The neutron and gamma doses were measured separately with thermoluminescent detectors. In this work, we have also demonstrated usefulness of a new system for positioning and removing samples in channel BH#3 of the IEA-R1 reactor at the Instituto de Pesquisas Energéticas e Nucleares (Brazil) without necessity of interrupting the reactor operation.

PMID:
21075641
DOI:
10.1016/j.apradiso.2010.10.025
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center