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Microorganisms. 2019 Oct 29;7(11). pii: E504. doi: 10.3390/microorganisms7110504.

Comparative Study on A Novel Pathogen of European Seabass. Diversity of Aeromonas veronii in the Aegean Sea.

Author information

1
Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, 71500 Crete, Greece. msmyrli@hcmr.gr.
2
Department of Biology, University of Crete, Heraklion, 70013 Crete, Greece. msmyrli@hcmr.gr.
3
Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, 71500 Crete, Greece. triga@hcmr.gr.
4
Department of Biology, University of Crete, Heraklion, 70013 Crete, Greece. triga@hcmr.gr.
5
Fish Pathology Department, Selonda Aquaculture, 15125 Athens, Greece. adourala@otenet.gr.
6
VETCARETM, 11528 Athens, Greece. info@vetcare.gr.
7
Department of Biology, University of Crete, Heraklion, 70013 Crete, Greece. pavlidis@biology.uoc.gr.
8
Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, 71500 Crete, Greece. qvha@hcmr.gr.
9
Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, 71500 Crete, Greece. katharios@hcmr.gr.

Abstract

Aeromonas veronii is an emerging pathogen causing severe pathology and mortalities in European seabass aquaculture in the Aegean Sea, Mediterranean. More than 50 strains of the pathogen were characterized biochemically and genetically in order to study the epidemiology of the disease, as well as the phylogeny and virulence of the bacterium. Based on the phenotypic characteristics, the isolates form three groups consisting of: (a) the West Aegean Sea, non-motile, non-pigment-producing strains, (b) the West Aegean Sea, motile, and pigment-producing strains and (c) the East Aegean Sea motile strains that produce minute amounts of pigment. All strains were highly similar at the genomic level; however, the pattern of West/East geographic origin was reflected in biochemical properties, in general genomic level comparison and in the putative virulent factors studied. Type VI secretion system was not detected in the western strains. The outer membrane protein (OMP) profile which contains proteins that are putative antigenic factors, was very similar between strains from the different areas. Although most of the OMPs were detected in all strains with great sequence similarity, diversification according to geographic origin was evident in known antigenic factors such as the maltoporin LamB. A systematic comparative analysis of the strains is presented and discussed in view of the emergence of A. veronii as a significant pathogen for the Mediterranean aquaculture.

KEYWORDS:

Aegean Sea; Aeromonas veronii; European seabass; Mediterranean; OMPs; comparative genomics; virulence

PMID:
31671797
DOI:
10.3390/microorganisms7110504
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