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Items: 1 to 20 of 29


Calcium-Binding Generates the Semi-Clathrate Waters on a Type II Antifreeze Protein to Adsorb onto an Ice Crystal Surface.

Arai T, Nishimiya Y, Ohyama Y, Kondo H, Tsuda S.

Biomolecules. 2019 Apr 27;9(5). pii: E162. doi: 10.3390/biom9050162.


Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein.

Yamazaki A, Nishimiya Y, Tsuda S, Togashi K, Munehara H.

Biomolecules. 2019 Apr 6;9(4). pii: E139. doi: 10.3390/biom9040139.


Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein.

Mahatabuddin S, Fukami D, Arai T, Nishimiya Y, Shimizu R, Shibazaki C, Kondo H, Adachi M, Tsuda S.

Proc Natl Acad Sci U S A. 2018 May 22;115(21):5456-5461. doi: 10.1073/pnas.1800635115. Epub 2018 May 7.


Concentration-dependent oligomerization of an alpha-helical antifreeze polypeptide makes it hyperactive.

Mahatabuddin S, Hanada Y, Nishimiya Y, Miura A, Kondo H, Davies PL, Tsuda S.

Sci Rep. 2017 Feb 13;7:42501. doi: 10.1038/srep42501.


Induction of Glycosphingolipid GM3 Expression by Valproic Acid Suppresses Cancer Cell Growth.

Kawashima N, Nishimiya Y, Takahata S, Nakayama KI.

J Biol Chem. 2016 Oct 7;291(41):21424-21433. Epub 2016 Aug 18.


Prolonging hypothermic storage (4 C) of bovine embryos with fish antifreeze protein.

Ideta A, Aoyagi Y, Tsuchiya K, Nakamura Y, Hayama K, Shirasawa A, Sakaguchi K, Tominaga N, Nishimiya Y, Tsuda S.

J Reprod Dev. 2015;61(1):1-6. doi: 10.1262/jrd.2014-073. Epub 2014 Oct 10.


Hyperactive antifreeze protein from an Antarctic sea ice bacterium Colwellia sp. has a compound ice-binding site without repetitive sequences.

Hanada Y, Nishimiya Y, Miura A, Tsuda S, Kondo H.

FEBS J. 2014 Aug;281(16):3576-90. doi: 10.1111/febs.12878. Epub 2014 Jul 4.


Determining the ice-binding planes of antifreeze proteins by fluorescence-based ice plane affinity.

Basu K, Garnham CP, Nishimiya Y, Tsuda S, Braslavsky I, Davies P.

J Vis Exp. 2014 Jan 15;(83):e51185. doi: 10.3791/51185.


Antifreeze protein prolongs the life-time of insulinoma cells during hypothermic preservation.

Kamijima T, Sakashita M, Miura A, Nishimiya Y, Tsuda S.

PLoS One. 2013 Sep 17;8(9):e73643. doi: 10.1371/journal.pone.0073643. eCollection 2013.


A simple medium enables bovine embryos to be held for seven days at 4°C.

Ideta A, Aoyagi Y, Tsuchiya K, Kamijima T, Nishimiya Y, Tsuda S.

Sci Rep. 2013;3:1173. doi: 10.1038/srep01173. Epub 2013 Jan 30.


NMR structure note: a defective isoform and its activity-improved variant of a type III antifreeze protein from Zoarces elongates Kner.

Kumeta H, Ogura K, Nishimiya Y, Miura A, Inagaki F, Tsuda S.

J Biomol NMR. 2013 Feb;55(2):225-30. doi: 10.1007/s10858-012-9703-9. Epub 2013 Jan 4. No abstract available.


Engineering a naturally inactive isoform of type III antifreeze protein into one that can stop the growth of ice.

Garnham CP, Nishimiya Y, Tsuda S, Davies PL.

FEBS Lett. 2012 Nov 2;586(21):3876-81. doi: 10.1016/j.febslet.2012.09.017. Epub 2012 Sep 24.


Synthetic study and structural analysis of the antifreeze agent xylomannan from Upis ceramboides.

Ishiwata A, Sakurai A, Nishimiya Y, Tsuda S, Ito Y.

J Am Chem Soc. 2011 Dec 7;133(48):19524-35. doi: 10.1021/ja208528c. Epub 2011 Nov 14.


Comparison of functional properties of two fungal antifreeze proteins from Antarctomyces psychrotrophicus and Typhula ishikariensis.

Xiao N, Suzuki K, Nishimiya Y, Kondo H, Miura A, Tsuda S, Hoshino T.

FEBS J. 2010 Jan;277(2):394-403. doi: 10.1111/j.1742-4658.2009.07490.x. Epub 2009 Dec 18.


Construction of time-lapse scanning electrochemical microscopy with temperature control and its application to evaluate the preservation effects of antifreeze proteins on living cells.

Hirano Y, Nishimiya Y, Kowata K, Mizutani F, Tsuda S, Komatsu Y.

Anal Chem. 2008 Dec 1;80(23):9349-54. doi: 10.1021/ac8018334.


Fully active QAE isoform confers thermal hysteresis activity on a defective SP isoform of type III antifreeze protein.

Takamichi M, Nishimiya Y, Miura A, Tsuda S.

FEBS J. 2009 Mar;276(5):1471-9. doi: 10.1111/j.1742-4658.2009.06887.x.


Crystal structure and mutational analysis of Ca2+-independent type II antifreeze protein from longsnout poacher, Brachyopsis rostratus.

Nishimiya Y, Kondo H, Takamichi M, Sugimoto H, Suzuki M, Miura A, Tsuda S.

J Mol Biol. 2008 Oct 10;382(3):734-46. doi: 10.1016/j.jmb.2008.07.042. Epub 2008 Jul 22.


Hypothermic preservation effect on mammalian cells of type III antifreeze proteins from notched-fin eelpout.

Hirano Y, Nishimiya Y, Matsumoto S, Matsushita M, Todo S, Miura A, Komatsu Y, Tsuda S.

Cryobiology. 2008 Aug;57(1):46-51. doi: 10.1016/j.cryobiol.2008.05.006. Epub 2008 Jun 4.


Two domains of RD3 antifreeze protein diffuse independently.

Holland NB, Nishimiya Y, Tsuda S, Sönnichsen FD.

Biochemistry. 2008 Jun 3;47(22):5935-41. doi: 10.1021/bi8001924. Epub 2008 May 7.


Effect of annealing time of an ice crystal on the activity of type III antifreeze protein.

Takamichi M, Nishimiya Y, Miura A, Tsuda S.

FEBS J. 2007 Dec;274(24):6469-76. Epub 2007 Nov 19.

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