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Sensors (Basel). 2018 Oct 29;18(11). pii: E3672. doi: 10.3390/s18113672.

Time Sequential Single-Cell Patterning with High Efficiency and High Density.

Author information

1
State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University, Beijing 100084, China. ly-15@tsinghua.org.cn.
2
State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University, Beijing 100084, China. rendh@tsinghua.edu.cn.
3
State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University, Beijing 100084, China. xixin.ling@ptpcapital.com.
4
State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University, Beijing 100084, China. liang-wb12@tsinghua.org.cn.
5
State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University, Beijing 100084, China. lijing18@mails.tsinghua.edu.cn.
6
State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University, Beijing 100084, China. yz-dpi@tsinghua.edu.cn.
7
Center for Biosystems Dynamics Research (BDR), RIKEN, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan. yaxiaer.yalikun@riken.jp.
8
Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan. yaxiaer.yalikun@riken.jp.
9
Center for Biosystems Dynamics Research (BDR), RIKEN, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan. yo.tanaka@riken.jp.

Abstract

Single-cell capture plays an important role in single-cell manipulation and analysis. This paper presents a microfluidic device for deterministic single-cell trapping based on the hydrodynamic trapping mechanism. The device is composed of an S-shaped loop channel and thousands of aligned trap units. This arrayed structure enables each row of the device to be treated equally and independently, as it has row periodicity. A theoretical model was established and a simulation was conducted to optimize the key geometric parameters, and the performance was evaluated by conducting experiments on MCF-7 and Jurkat cells. The results showed improvements in single-cell trapping ability, including loading efficiency, capture speed, and the density of the patterned cells. The optimized device can achieve a capture efficiency of up to 100% and single-cell capture efficiency of up to 95%. This device offers 200 trap units in an area of 1 mm², which enables 100 single cells to be observed simultaneously using a microscope with a 20× objective lens. One thousand cells can be trapped sequentially within 2 min; this is faster than the values obtained with previously reported devices. Furthermore, the cells can also be recovered by reversely infusing solutions. The structure can be easily extended to a large scale, and a patterned array with 32,000 trap sites was accomplished on a single chip. This device can be a powerful tool for high-throughput single-cell analysis, cell heterogeneity investigation, and drug screening.

KEYWORDS:

cell patterning; cell trapping; lab-on-a-chip; microfluidics; single-cell analysis

PMID:
30380644
PMCID:
PMC6264106
DOI:
10.3390/s18113672
[Indexed for MEDLINE]
Free PMC Article

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