Format

Send to

Choose Destination
Microbiol Res. 2016 Nov;192:300-312. doi: 10.1016/j.micres.2016.08.007. Epub 2016 Aug 6.

Bio-protective microbial agents from rhizosphere eco-systems trigger plant defense responses provide protection against sheath blight disease in rice (Oryza sativa L.).

Author information

1
ICAR-National Bureau of Agriculturally Important Microorganisms, Kushmaur, Maunath Bhanjan 275103, India. Electronic address: udaiars.nbaim@gmail.com.
2
ICAR-National Bureau of Agriculturally Important Microorganisms, Kushmaur, Maunath Bhanjan 275103, India.
3
Department of Plant Pathology, College of Agriculture, Narendra Dev University of Agriculture and Technology, Kumarganj, Faizabad 224229, India.
4
Department of Bioscience, Faculty of Applied Science, Integral University, Lucknow 226026, India.
5
Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India.
6
Department of Mycology and Plant Pathology (Krishi Vigyan Kendra), Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India.
7
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India.
8
ICAR-Directorate of Seed Research, Kushmaur, Maunath Bhanjan 275103, India.
9
Division of Soil Biology, Indian Institute of Soil Science, Nabibagh, Berasia road, Bhopal 462038, India.

Abstract

Sheath blight of rice (Oryza sativa L.) caused by Rhizoctonia solani is a major disease and attempts are being made to develop microbe based technologies for biocontrol of this pathogen. However, the mechanisms of biocontrol are not fully understood and still require indepth study in the backdrop of emerging concepts in biological systems. The present investigation was aimed at deciphering the mechanisms of biocontrol of sheath blight of rice employing Pseudomonas fluorescens and Trichoderma harzianum as model agents for biocontrol. Initially 25, 5 and 5 strains of P. fluorescens, T. viride and T. harzianum, respectively, were screened for their biocontrol potential. Out of which, six strains with higher value of percent inhibition of fungal mycelium in dual plate assay were selected. The role of P. fluorescens, T. viride and T. harzianum were investigated in induction and bioaccumulation of natural antioxidants, defence-related biomolecules and other changes in plant which lead not only to growth promotion but also protection from pathogenic stress conditions in rice. The two most promising strains, P. fluorescens PF-08 and T. harzianum UBSTH-501 selected on the basis of in planta evaluation, when applied individually or in combination, significantly enhanced the accumulation of defence-related biomolecules, enzymes and exhibited biocontrol potential against R. solani. A modified/newly developed delivery system was applied for the first time in the experiments involving inoculation of plants with both bioagents, viz. P. fluorescens PF-08 and T. harzianum UBSTH-501. Results suggested that application of P. fluorescens PF-08 and T. harzianum UBSTH-501 alone or in combination, not only helps in control of the disease but also increases plant growth along with reduction in application of toxic chemical pesticides.

KEYWORDS:

Defence-related biomolecules; Induced systemic resistance (ISR); Pseudomonas; Rhizosphere; Signalling biomolecules; Trichoderma

PMID:
27664749
DOI:
10.1016/j.micres.2016.08.007
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center