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High Throughput. 2019 Feb 13;8(1). pii: E5. doi: 10.3390/ht8010005.

A Pilot Study to Investigate the Balance between Proteases and α1-Antitrypsin in Bronchoalveolar Lavage Fluid of Lung Transplant Recipients.

Author information

1
Department of Molecular Medicine, Biochemistry Unit, University of Pavia, 27100 Pavia, Italy. maddalena.cagnone@gmail.com.
2
Department of Internal Medicine and Therapeutics, Section of Pneumology, University of Pavia, 27100 Pavia, Italy. davidepiloni@live.it.
3
IRCCS Foundation Policlinico San Matteo, Department of Medical Sciences and Infective Diseases, Unit of Respiratory Diseases, 27100 Pavia, Italy. davidepiloni@live.it.
4
Department of Internal Medicine and Therapeutics, Section of Pneumology, University of Pavia, 27100 Pavia, Italy. I.Ferrarotti@smatteo.pv.it.
5
Department of Molecular Medicine, Biochemistry Unit, University of Pavia, 27100 Pavia, Italy. monica.divenere01@universitadipavia.it.
6
Department of Molecular Medicine, Biochemistry Unit, University of Pavia, 27100 Pavia, Italy. simona.viglio@unipv.it.
7
IRCCS Foundation Policlinico San Matteo, Department of Medical Sciences and Infective Diseases, Unit of Respiratory Diseases, 27100 Pavia, Italy. sara.magni04@gmail.com.
8
Department of Molecular Medicine, Biochemistry Unit, University of Pavia, 27100 Pavia, Italy. abardoni@unipv.it.
9
Department of Molecular Medicine, Biochemistry Unit, University of Pavia, 27100 Pavia, Italy. roberta.salvini@unipv.it.
10
Department of Biology and Biotechnologies "L. Spallanzani″, Biochemistry Unit, University of Pavia, 27100 Pavia, Italy. marco.fumagalli@unipv.it.
11
Department of Biology and Biotechnologies "L. Spallanzani″, Biochemistry Unit, University of Pavia, 27100 Pavia, Italy. piadarol@unipv.it.
12
Department of Internal Medicine and Therapeutics, Section of Pneumology, University of Pavia, 27100 Pavia, Italy. sabrinamartinello@hotmail.it.
13
Department of Internal Medicine and Therapeutics, Section of Pneumology, University of Pavia, 27100 Pavia, Italy. f.meloni@smatteo.pv.it.
14
IRCCS Foundation Policlinico San Matteo, Department of Medical Sciences and Infective Diseases, Unit of Respiratory Diseases, 27100 Pavia, Italy. f.meloni@smatteo.pv.it.

Abstract

: The neutrophilic component in bronchiolitis obliterans syndrome (BOS, the main form of chronic lung rejection), plays a crucial role in the pathogenesis and maintenance of the disorder. Human Neutrophil Elastase (HNE), a serine protease responsible of elastin degradation whose action is counteracted by α1-antitrypsin (AAT), a serum inhibitor specific for this protease. This work aimed to investigate the relationship between HNE and AAT in bronchoalveolar lavage fluid (BALf) from stable lung transplant recipients and BOS patients to understand whether the imbalance between proteases and inhibitors is relevant to the development of BOS. To reach this goal a multidisciplinary procedure was applied which included: (i) the use of electrophoresis/western blotting coupled with liquid chromatography-mass spectrometric analysis; (ii) the functional evaluation of the residual antiprotease activity, and (iii) a neutrophil count.

KEYWORDS:

alpha 1-antitrypsin; bronchiolitis obliterans syndrome; bronchoalveolar lavage fluid; elastase; lung; neutrophils

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