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Items: 1 to 20 of 36


Probing the role of an invariant active site His in family GH1 β-glycosidases.

Strazzulli A, Perugino G, Mazzone M, Rossi M, Withers SG, Moracci M.

J Enzyme Inhib Med Chem. 2019 Dec;34(1):973-980. doi: 10.1080/14756366.2019.1608198.


The DNA Alkylguanine DNA Alkyltransferase-2 (AGT-2) Of Caenorhabditis Elegans Is Involved In Meiosis And Early Development Under Physiological Conditions.

Serpe M, Forenza C, Adamo A, Russo N, Perugino G, Ciaramella M, Valenti A.

Sci Rep. 2019 May 3;9(1):6889. doi: 10.1038/s41598-019-43394-1.


Thermostability enhancement of the α-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling-protein-tag system (ASLtag).

Del Prete S, Merlo R, Valenti A, Mattossovich R, Rossi M, Carginale V, Supuran CT, Perugino G, Capasso C.

J Enzyme Inhib Med Chem. 2019 Dec;34(1):946-954. doi: 10.1080/14756366.2019.1605991.


Optimization of an In Vitro Transcription/Translation System Based on Sulfolobus solfataricus Cell Lysate.

Lo Gullo G, Mattossovich R, Perugino G, La Teana A, Londei P, Benelli D.

Archaea. 2019 Feb 11;2019:9848253. doi: 10.1155/2019/9848253. eCollection 2019.


An AGT-based protein-tag system for the labelling and surface immobilization of enzymes on E. coli outer membrane.

Merlo R, Del Prete S, Valenti A, Mattossovich R, Carginale V, Supuran CT, Capasso C, Perugino G.

J Enzyme Inhib Med Chem. 2019 Dec;34(1):490-499. doi: 10.1080/14756366.2018.1559161.


Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with a fluorescent probe.

Rossi F, Morrone C, Massarotti A, Ferraris DM, Valenti A, Perugino G, Miggiano R.

Biochem Biophys Res Commun. 2018 Jun 7;500(3):698-703. doi: 10.1016/j.bbrc.2018.04.139. Epub 2018 Apr 30.


Structure and Properties of DNA Molecules Over The Full Range of Biologically Relevant Supercoiling States.

Bettotti P, Visone V, Lunelli L, Perugino G, Ciaramella M, Valenti A.

Sci Rep. 2018 Apr 18;8(1):6163. doi: 10.1038/s41598-018-24499-5.


Every OGT Is Illuminated … by Fluorescent and Synchrotron Lights.

Miggiano R, Valenti A, Rossi F, Rizzi M, Perugino G, Ciaramella M.

Int J Mol Sci. 2017 Dec 5;18(12). pii: E2613. doi: 10.3390/ijms18122613. Review.


In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag.

Visone V, Han W, Perugino G, Del Monaco G, She Q, Rossi M, Valenti A, Ciaramella M.

PLoS One. 2017 Oct 3;12(10):e0185791. doi: 10.1371/journal.pone.0185791. eCollection 2017.


Interdomain interactions rearrangements control the reaction steps of a thermostable DNA alkyltransferase.

Morrone C, Miggiano R, Serpe M, Massarotti A, Valenti A, Del Monaco G, Rossi M, Rossi F, Rizzi M, Perugino G, Ciaramella M.

Biochim Biophys Acta Gen Subj. 2017 Feb;1861(2):86-96. doi: 10.1016/j.bbagen.2016.10.020. Epub 2016 Oct 22.


Erratum to: A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA-alkyl-transferase by protein engineering.

Vettone A, Serpe M, Hidalgo A, Berenguer J, Del Monaco G, Valenti A, Rossi M, Ciaramella M, Perugino G.

Extremophiles. 2016 Jan;20(1):15-7. doi: 10.1007/s00792-015-0803-9. No abstract available.


Crystal structure of Mycobacterium tuberculosis O6-methylguanine-DNA methyltransferase protein clusters assembled on to damaged DNA.

Miggiano R, Perugino G, Ciaramella M, Serpe M, Rejman D, Páv O, Pohl R, Garavaglia S, Lahiri S, Rizzi M, Rossi F.

Biochem J. 2016 Jan 15;473(2):123-33. doi: 10.1042/BJ20150833. Epub 2015 Oct 28.


A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA- alkyl-transferase by protein engineering.

Vettone A, Serpe M, Hidalgo A, Berenguer J, del Monaco G, Valenti A, Rossi M, Ciaramella M, Perugino G.

Extremophiles. 2016 Jan;20(1):1-13. doi: 10.1007/s00792-015-0791-9. Erratum in: Extremophiles. 2016 Jan;20(1):15-7.


Structure-function relationships governing activity and stability of a DNA alkylation damage repair thermostable protein.

Perugino G, Miggiano R, Serpe M, Vettone A, Valenti A, Lahiri S, Rossi F, Rossi M, Rizzi M, Ciaramella M.

Nucleic Acids Res. 2015 Oct 15;43(18):8801-16. doi: 10.1093/nar/gkv774. Epub 2015 Jul 30.


Chromatin structure and dynamics in hot environments: architectural proteins and DNA topoisomerases of thermophilic archaea.

Visone V, Vettone A, Serpe M, Valenti A, Perugino G, Rossi M, Ciaramella M.

Int J Mol Sci. 2014 Sep 25;15(9):17162-87. doi: 10.3390/ijms150917162. Review.


Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase.

Vettone A, Perugino G, Rossi M, Valenti A, Ciaramella M.

Extremophiles. 2014 Sep;18(5):895-904. doi: 10.1007/s00792-014-0662-9. Epub 2014 Aug 8. Review.


The reverse gyrase from Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities.

Jamroze A, Perugino G, Valenti A, Rashid N, Rossi M, Akhtar M, Ciaramella M.

J Biol Chem. 2014 Feb 7;289(6):3231-43. doi: 10.1074/jbc.M113.517649. Epub 2013 Dec 17.


Biochemical and structural studies of the Mycobacterium tuberculosis O6-methylguanine methyltransferase and mutated variants.

Miggiano R, Casazza V, Garavaglia S, Ciaramella M, Perugino G, Rizzi M, Rossi F.

J Bacteriol. 2013 Jun;195(12):2728-36. doi: 10.1128/JB.02298-12. Epub 2013 Apr 5.


Synergic and opposing activities of thermophilic RecQ-like helicase and topoisomerase 3 proteins in Holliday junction processing and replication fork stabilization.

Valenti A, De Felice M, Perugino G, Bizard A, Nadal M, Rossi M, Ciaramella M.

J Biol Chem. 2012 Aug 31;287(36):30282-95. doi: 10.1074/jbc.M112.366377. Epub 2012 Jun 21.


Thermophilic glycosynthases for oligosaccharides synthesis.

Cobucci-Ponzano B, Perugino G, Strazzulli A, Rossi M, Moracci M.

Methods Enzymol. 2012;510:273-300. doi: 10.1016/B978-0-12-415931-0.00015-X.


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