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Items: 1 to 20 of 81

1.

Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells.

Blessing D, Vachey G, Pythoud C, Rey M, Padrun V, Wurm FM, Schneider BL, Déglon N.

Mol Ther Methods Clin Dev. 2018 Nov 22;13:14-26. doi: 10.1016/j.omtm.2018.11.004. eCollection 2019 Jun 14.

2.

Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line.

Shen X, Dojcinovic D, Baldi L, Hacker DL, Luescher IF, Wurm FM.

Biotechnol Lett. 2018 Jan;40(1):85-92. doi: 10.1007/s10529-017-2440-0. Epub 2017 Oct 9.

PMID:
28993910
3.

Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes.

Zhu LK, Song BY, Wang ZL, Monteil DT, Shen X, Hacker DL, De Jesus M, Wurm FM.

Biotechnol Prog. 2017 Jan;33(1):192-200. doi: 10.1002/btpr.2375. Epub 2016 Oct 31.

PMID:
27690196
4.

A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation.

Monteil DT, Juvet V, Paz J, Moniatte M, Baldi L, Hacker DL, Wurm FM.

Biotechnol Prog. 2016 Sep;32(5):1174-1180. doi: 10.1002/btpr.2328. Epub 2016 Aug 11.

PMID:
27453130
5.

Multigene expression in stable CHO cell pools generated with the piggyBac transposon system.

Balasubramanian S, Wurm FM, Hacker DL.

Biotechnol Prog. 2016 Sep;32(5):1308-1317. doi: 10.1002/btpr.2319. Epub 2016 Jul 13.

PMID:
27302570
6.

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension.

Monteil DT, Shen X, Tontodonati G, Baldi L, Hacker DL, Wurm FM.

Anal Biochem. 2016 Jul 15;505:26-8. doi: 10.1016/j.ab.2016.04.009. Epub 2016 Apr 27.

PMID:
27130502
7.

Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines.

Balasubramanian S, Rajendra Y, Baldi L, Hacker DL, Wurm FM.

Biotechnol Bioeng. 2016 Jun;113(6):1234-43. doi: 10.1002/bit.25888. Epub 2015 Dec 9.

PMID:
26616356
8.

A simple plasmid-based transient gene expression method using High Five cells.

Shen X, Pitol AK, Bachmann V, Hacker DL, Baldi L, Wurm FM.

J Biotechnol. 2015 Dec 20;216:67-75. doi: 10.1016/j.jbiotec.2015.10.007. Epub 2015 Oct 23.

PMID:
26476358
9.

Enhanced plasmid DNA utilization in transiently transfected CHO-DG44 cells in the presence of polar solvents.

Rajendra Y, Balasubramanian S, Kiseljak D, Baldi L, Wurm FM, Hacker DL.

Biotechnol Prog. 2015 Nov-Dec;31(6):1571-8. doi: 10.1002/btpr.2152. Epub 2015 Aug 25.

PMID:
26260195
10.

Production of active glycosylation-deficient γ-secretase complex for crystallization studies.

López AR, Dimitrov M, Gerber H, Braman V, Hacker DL, Wurm FM, Fraering PC.

Biotechnol Bioeng. 2015 Dec;112(12):2516-26. doi: 10.1002/bit.25675. Epub 2015 Jul 14.

PMID:
26059427
11.

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools.

Balasubramanian S, Matasci M, Kadlecova Z, Baldi L, Hacker DL, Wurm FM.

J Biotechnol. 2015 Apr 20;200:61-9. doi: 10.1016/j.jbiotec.2015.03.001. Epub 2015 Mar 7.

PMID:
25758242
12.

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells.

Rajendra Y, Kiseljak D, Baldi L, Wurm FM, Hacker DL.

Biotechnol Prog. 2015 Mar-Apr;31(2):541-9. doi: 10.1002/btpr.2064. Epub 2015 Feb 26.

PMID:
25683738
13.

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells.

Davami F, Baldi L, Rajendra Y, M Wurm F.

Int J Mol Cell Med. 2014 Summer;3(3):146-56.

14.

Virus-free transient protein production in Sf9 cells.

Shen X, Hacker DL, Baldi L, Wurm FM.

J Biotechnol. 2014 Feb 10;171:61-70. doi: 10.1016/j.jbiotec.2013.11.018. Epub 2013 Dec 11.

PMID:
24333123
15.

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells.

Hacker DL, Kiseljak D, Rajendra Y, Thurnheer S, Baldi L, Wurm FM.

Protein Expr Purif. 2013 Nov;92(1):67-76. doi: 10.1016/j.pep.2013.09.001. Epub 2013 Sep 8.

PMID:
24021764
16.

DNA delivery with hyperbranched polylysine: a comparative study with linear and dendritic polylysine.

Kadlecova Z, Rajendra Y, Matasci M, Baldi L, Hacker DL, Wurm FM, Klok HA.

J Control Release. 2013 Aug 10;169(3):276-88. doi: 10.1016/j.jconrel.2013.01.019. Epub 2013 Feb 1.

PMID:
23379996
17.

Highly efficient production of the Alzheimer's γ-secretase integral membrane protease complex by a multi-gene stable integration approach.

Alattia JR, Matasci M, Dimitrov M, Aeschbach L, Balasubramanian S, Hacker DL, Wurm FM, Fraering PC.

Biotechnol Bioeng. 2013 Jul;110(7):1995-2005. doi: 10.1002/bit.24851. Epub 2013 Feb 15.

PMID:
23359429
18.

A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells.

Michel PO, Degen C, Hubert M, Baldi L, Hacker DL, Wurm FM.

Anal Biochem. 2012 Nov 15;430(2):138-40. doi: 10.1016/j.ab.2012.08.028. Epub 2012 Sep 6.

PMID:
22960013
19.

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity.

Zagari F, Jordan M, Stettler M, Broly H, Wurm FM.

N Biotechnol. 2013 Jan 25;30(2):238-45. doi: 10.1016/j.nbt.2012.05.021. Epub 2012 Jun 6.

PMID:
22683938
20.

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells.

Rajendra Y, Kiseljak D, Manoli S, Baldi L, Hacker DL, Wurm FM.

Biotechnol Bioeng. 2012 Sep;109(9):2271-8. doi: 10.1002/bit.24494. Epub 2012 Mar 30.

PMID:
22422519

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