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Sensors (Basel). 2019 Jan 21;19(2). pii: E436. doi: 10.3390/s19020436.

Experimental and Numerical Design and Evaluation of a Vibration Bioreactor using Piezoelectric Patches.

Author information

1
Center for Industrial Diagnostics and Fluid Dynamics (CDIF), Polytechnic University of Catalonia (UPC), 08034 Barcelona, Spain. david.valentin@upc.edu.
2
Experimental Trauma Surgery, Justus Liebig University of Giessen, Germany. Charline.Roehr@med.uni-giessen.de.
3
Center for Industrial Diagnostics and Fluid Dynamics (CDIF), Polytechnic University of Catalonia (UPC), 08034 Barcelona, Spain. alexpresas@hotmail.com.
4
Department of Energy and Power Engineering, Tsinghua University, Beijing 100084, China. alexpresas@hotmail.com.
5
Experimental Trauma Surgery, Justus Liebig University of Giessen, Germany. christian.heiss@chiru.med.uni-giessen.de.
6
Center for Industrial Diagnostics and Fluid Dynamics (CDIF), Polytechnic University of Catalonia (UPC), 08034 Barcelona, Spain. eduard.egusquiza@upc.edu.
7
Experimental Trauma Surgery, Justus Liebig University of Giessen, Germany. wolfram.bosbach@med.uni-giessen.de.

Abstract

In this present study, we propose a method for exposing biological cells to mechanical vibration. The motive for our research was to design a bioreactor prototype in which in-depth in vitro studies about the influence of vibration on cells and their metabolism can be performed. The therapy of cancer or antibacterial measures are applications of interest. In addition, questions about the reaction of neurons to vibration are still largely unanswered. In our methodology, we used a piezoelectric patch (PZTp) for inducing mechanical vibration to the structure. To control the vibration amplitude, the structure could be excited at different frequency ranges, including resonance and non-resonance conditions. Experimental results show the vibration amplitudes expected for every frequency range tested, as well as the vibration pattern of those excitations. These are essential parameters to quantify the effect of vibration on cell behavior. Furthermore, a numerical model was validated with the experimental results presenting accurate results for the prediction of those parameters. With the calibrated numerical model, we will study in greater depth the effects of different vibration patterns for the abovementioned cell types.

KEYWORDS:

PZTp; bioreactor; cell biology; finite element method; laser Doppler vibrometer (LDV); ultrasound; vibration

PMID:
30669693
PMCID:
PMC6359548
DOI:
10.3390/s19020436
[Indexed for MEDLINE]
Free PMC Article

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