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J Magn Reson. 1999 Feb;136(2):211-3.

Offset profiles of selective pulses in isotopically labeled macromolecules.

Author information

1
Section de Chimie, Université de Lausanne, BCH, Lausanne, 1015, Switzerland.

Abstract

The experimental verification of offset profiles and calibration of selective pulses in NMR is usually carried out with doped water samples but not under conditions typical of macromolecules with short T2, long T1, and possibly homo- and heteronuclear couplings. A new method for selective excitation in isotopically labeled macromolecules is shown to be particularly suited to this purpose. This is illustrated for a backbone amide resonance in a sample of 15N-labeled human ubiquitin.

PMID:
9986763
DOI:
10.1006/jmre.1998.1623
[Indexed for MEDLINE]

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