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Gene Ther. 1998 Nov;5(11):1481-7.

Gene transfer vectors derived from equine infectious anemia virus.

Author information

1
Department of Medicine, University of North Carolina at Chapel Hill, USA.

Abstract

Equine infectious anemia virus (EIAV) is a lentivirus in the retrovirus family of viruses. Replication-defective EIAV vectors have been constructed that encode bacterial puromycin-N-acetyl transferase and E. coli beta-galactosidase. These vectors could be prepared with titers greater than 10(5) infectious units/ml and were able to act as vehicles to carry genes into cultured human cells. In addition, stable helper cell lines were created by modifying human 293 cells to express EIAV proteins. Unlike retroviral vectors based on murine leukemia virus, EIAV lentiviral vectors transduce nondividing (aphidicolin-arrested) cells. These properties make EIAV vectors promising gene transfer vehicles.

PMID:
9930301
DOI:
10.1038/sj.gt.3300768
[Indexed for MEDLINE]
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