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EMBO J. 1999 Feb 1;18(3):565-77.

Identification of MINUS, a small polypeptide that functions as a microtubule nucleation suppressor.

Author information

1
Department of Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, Germany.

Abstract

In eukaryotic cells, tubulin polymerization must be regulated precisely during cell division and differentiation. To identify new mechanisms involved in cellular microtubule formation, we isolated an activity that suppresses microtubule nucleation in vitro. The activity was due to a small acidic polypeptide of 4.7 kDa which we named MINUS (microtubule nucleation suppressor). MINUS inhibited tau- and taxol-mediated microtubule assembly in vitro and was inactivated by dephosphorylation. The protein was purified to homogeneity from cultured neural (PC12) cells and bovine brain. Microinjection of MINUS caused a transient loss of dynamic microtubules in Vero cells. The results suggest that MINUS acts with a novel mechanism on tubulin polymerization, thus regulating microtubule formation in living cells.

PMID:
9927416
PMCID:
PMC1171149
DOI:
10.1093/emboj/18.3.565
[Indexed for MEDLINE]
Free PMC Article

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