Send to

Choose Destination
See comment in PubMed Commons below
Mol Ecol. 1999 Jan;8(1):37-45.

Intrasporal variability of ribosomal sequences in the endomycorrhizal fungus Gigaspora margarita.

Author information

  • 1Dipartimento di Biologia Vegetale dell'Universit√† and Centro di Studio sulla Micologia del Terreno del Consiglio Nazionale delle Ricerche, Torino, Italy.


The sequence variability of the ribosomal internal transcribed spacer (ITS) region, which comprises the 5.8 gene and the flanking regions ITS1 and ITS2, was investigated in the arbuscular mycorrhizal fungus Gigaspora margarita. DNA analysis of a multispore preparation and three single spores led to the identification of 11 slightly different sequences (three variants within a single spore), indicating substantial intersporal and intrasporal genetic variability (up to 9% sequence divergence). The sequence variations inside a single spore may be higher than that observed between spores. Even so, primers designed on the ITS1 and ITS2 regions identified Gi. margarita isolates and detected the endophyte during colonization.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Write to the Help Desk