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Dev Genes Evol. 1999 Jan;209(1):63-8.

Green fluorescent proteins with short half-lives as reporters in Dictyostelium discoideum.

Author information

1
Zoologisches Institut, Ludwig-Maximilians-Universit√§t, Luisenstrasse 14, D-80333 M√ľnchen, Germany.

Abstract

We describe two modifications of the popular reporter green fluorescent protein (GFP) which have short half-lives in our system, the cellular slime mould Dictyostelium discoideum. One of these bears an N-terminal ubiquitin; this GFP was originally planned to be a substrate of the "N-end-rule" pathway, but deubiquitination does not seem to occur, and a degradation by the UFD (ubiquitin-fusion-degradation pathway seems more probable. The protein half-life is about 3-5 h. The second construct has an N-terminus derived from the L11 ribosomal protein; it is transported to the nucleus and broken down much more rapidly than the ubiquitin fusion (protein half-life about 30 min). We show examples of the use of these reporters in the study of gene expression in Dictyostelium.

PMID:
9914420
DOI:
10.1007/s004270050228
[Indexed for MEDLINE]

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