Format

Send to

Choose Destination
Virology. 1999 Jan 5;253(1):1-7.

De novo initiation of RNA synthesis by a recombinant flaviviridae RNA-dependent RNA polymerase.

Author information

1
Department of Biology, Indiana University, Bloomington, Indiana, 47405, USA.

Abstract

Recombinant RNA-dependent RNA polymerases have been reported to synthesize RNAs by extending from the 3' hydroxyl of a template or an oligonucleotide primer. De novo initiation has not been reported. Establishment of such an assay would facilitate the analysis of the initiation requirements and allow the testing of antiviral compounds specifically targeting initiation. Using chemically synthesized RNAs and DNAs, we demonstrate that the recombinant RNA-dependent RNA polymerase (NS5B) of bovine viral diarrhea virus initiates de novo RNA synthesis. Nucleotides required for efficient initiation of RNA synthesis and for stable interaction with NS5B were identified.

PMID:
9887312
DOI:
10.1006/viro.1998.9517
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center