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Int J Radiat Biol. 1998 Dec;74(6):793-8.

Studies of bystander effects in human fibroblasts using a charged particle microbeam.

Author information

1
Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, Middlesex, UK. Michael@graylab.ac.uk

Abstract

PURPOSE:

To determine the role of cell-to-cell mediated effects in the response of cells to different radiations by using a charged particle microbeam capable of targeting individual cells with counted particles.

MATERIALS AND METHODS:

Primary human fibroblasts were irradiated conventionally with X-rays or alpha-particles and scored for the induction of micronucleated or apoptotic cell formation at various times after irradiation. Cells were also individually irradiated with helium-3 particles from a microbeam and the distribution of damaged cells between hit and non-hit cells scored.

RESULTS:

Conventionally exposed fibroblasts showed a dose-dependent production of micronucleated and apoptotic cells 3 days after irradiation for both X-rays and alpha-particles, with a high RBE value for alpha-particles at low doses. Targeting individual alpha-particles, using a microbeam, to four cells within a population produced more micronucleated and apoptotic cells than expected on the basis of a direct effect only.

CONCLUSIONS:

This study provides direct evidence for the production of transmissible, cell-to-cell effects between hit and non-hit cells individually exposed to charged particles.

PMID:
9881726
DOI:
10.1080/095530098141087
[Indexed for MEDLINE]

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