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FEBS Lett. 1998 Dec 11;441(1):6-10.

The CGRP receptor can couple via pertussis toxin sensitive and insensitive G proteins.

Author information

1
Receptor Systems, Glaxo Wellcome Research Ltd., Medicines Research Centre, Stevenage, Hertfordshire, UK.

Abstract

Swiss 3T3 cell lines were constructed co-expressing receptor activity modifying protein (RAMP) 1 with the calcitonin receptor-like receptor (CRLR), and showed 125I-calcitonin-gene-related peptide (CGRP) 1 binding indicative of a type I CGRP receptor. Application of CGRP1 led to an increase in cAMP, which in 2/5 cell lines was augmented following pertussis toxin (PTX) pre-treatment. In Xenopus oocytes, expression of RAMP1, which potentiates an endogenous CGRP receptor, led to constitutive activation of co-expressed GIRK potassium channels. This potassium current was increased following CGRP application or co-expression of CRLR, but decreased by PTX or co-expression of transducin. We conclude that the CGRP receptor can signal to both PTX sensitive and insensitive G proteins.

PMID:
9877154
DOI:
10.1016/s0014-5793(98)01507-5
[Indexed for MEDLINE]
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