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Appl Environ Microbiol. 1999 Jan;65(1):346-50.

Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout.

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1
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.

Abstract

A PCR-based method was developed for the specific detection of Yersinia ruckeri in tissues of inoculated trout and naturally infected trout. No amplification products were obtained with other yersiniae, bacterial fish pathogens, or phylogenetically related bacteria (n = 34). The sensitivity of PCR detection was 60 to 65 bacterial cells per PCR tube, which was decreased to 10 to 20 cells by hybridization with a nonradioactive probe. The PCR assay proved to be as reliable as and faster than the conventional culture method for the detection of Y. ruckeri in infected trout tissues.

PMID:
9872807
PMCID:
PMC91030
[Indexed for MEDLINE]
Free PMC Article
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