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Infect Control Hosp Epidemiol. 1998 Dec;19(12):918-23.

Detection of Bordetella pertussis and respiratory synctial virus in air samples from hospital rooms.

Author information

1
University of California, Children's Hospital and Health Center, San Diego, USA.

Abstract

OBJECTIVE:

To evaluate the distribution of Bordetella pertussis and respiratory syncytial virus (RSV) in the hospital setting.

DESIGN:

Air samples were collected using filters in the hospital rooms of 12 children with pertussis and 27 children with RSV infection. Material eluted from these filters was subjected to RSV- and B pertussis-specific polymerase chain reaction (PCR) amplification.

SETTING:

Patients were hospitalized in private rooms in one of two referral centers, a university teaching hospital and a university-affiliated private children's hospital.

PATIENTS:

12 children (16 days-3 years of age) with documented pertussis infection and 27 patients (10 days-7 years of age) with documented RSV infection.

RESULTS:

B pertussis DNA was detected in 7 (58%) of 12 rooms housing pertussis patients and in 16 (25%) of 63 total samples. B pertussis DNA was detected as far as 4 m away from the patient's bedside. The detection of B pertussis DNA in air samples did not change over the short duration of hospitalization. RSV RNA was detected in 17 (63%) of 27 rooms housing RSV-infected patients and in 32 (22%) of 143 total samples. RSV RNA was detected at distances as far as 7 m from the patient's bedside and for up to 7 days of hospitalization.

CONCLUSIONS:

Using PCR-based detection methods, B pertussis DNA and RSV RNA both can be detected in air samples from the hospital rooms of infected patients. Both can be detected at large distances from a patient's bedside in a minority of cases. These detection methods are suitable for further studies of control measures used to contain nosocomial infections caused by both B pertussis and RSV.

PMID:
9872529
[Indexed for MEDLINE]

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