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Nucleic Acids Res. 1999 Jan 15;27(2):537-42.

A DNA polymerase stop assay for G-quadruplex-interactive compounds.

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Program in Molecular Biology, Division of Medicinal Chemistry and Drug Dynamics Institute, College of Pharmacy, The University of Texas at Austin, Austin, TX 78712, USA.


We have developed and characterized an assay for G-quadruplex-interactive compounds that makes use of the fact that G-rich DNA templates present obstacles to DNA synthesis by DNA polymerases. Using Taq DNA polymerase and the G-quadruplex binding 2, 6-diamidoanthraquinone BSU-1051, we find that BSU-1051 leads to enhanced arrest of DNA synthesis in the presence of K+by stabilizing an intramolecular G-quadruplex structure formed by four repeats of either TTGGGG or TTAGGG in the template strand. The data provide additional evidence that BSU-1051 modulates telomerase activity by stabilization of telomeric G-quadruplex DNA and point to a polymerase arrest assay as a sensitive method for screening for G-quadruplex-interactive agents with potential clinical utility.

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